The Resistance And Resistance Of Antibiotics Essay
Antibiotics were initially thought of as a superior drug that could kill many bacterial diseases. Prior to antibiotics, it was hard for anyone to fight a bacteria. However, bacteria are very changing and have begun being able to gain resistance against antibiotics due to overuse, not strong enough dosage of antibiotics, or improper use. By determining the resistance and cross-resistance, one may determine the the relative ability for bacteria to gain resistance to antibiotics. We hypothesis that if we expose Enterobacter cloacae to Tetracycline over a week, then the bacteria will evolve a resistance and the zone of inhibition will decrease. Also, the bacteria will gain a resistance to other antibiotics and biocides, such as Triclosan.
Our experiment was designed to show if E. cloacae develops resistance to tetracycline and cross-resistance to triclosan in a eight day period. To study resistance, we made bacterial lawns, broth tubes, and measured zone of inhibitions in millimeters. Where, to study cross-resistance, we made bacterial lawns and measured zone of inhibitions in millimeters. For standard procedure, reference (Department of Biology. 2015).
When exposed to tetracycline the mean zone of inhibition across the five replicates for the selected, E. cloacae bacteria (5.8mm +/- 0.97SE) was smaller than the parental control strain (11.2mm +/- 0.8SE; Figure 1). This difference in the means was statistically significant (p=0.0086, df=4, t=4.81).…