Enterobacter cloacae

antibiotics, or improper use. By determining the resistance and cross-resistance, one may determine the the relative ability for bacteria to gain resistance to antibiotics. We hypothesis that if we expose Enterobacter cloacae to Tetracycline over a week, then the bacteria will evolve a resistance and the zone of inhibition will decrease. Also, the bacteria will gain a resistance to other antibiotics and biocides, such as Triclosan.…

1) Alcaligenes faecalis is a Gram-negative coccobacilli that is motile, oxidase positive, catalase positive, non-spore-forming, non-fermenting, slow growing, and citrate positive obligate aerobe (Mahon et al., 2014) (Sachdeva et al., 1963). Its colony morphology is flat, thin, and rough with irregular edging (Mahon et al., 2014) and the flagella varies between the usual shape versus the curly structure, however, both shapes do not exist on the same individual bacteria (Leifson, 1960). The…

E. cloacae is a gram-negative, rod-shaped (bacilli) bacterium. It possesses flagella for motility and is a facultative anaerobic microorganism that is able to obtain energy from both aerobic and anaerobic environments (Britannica). E. cloacae is a nosocomial pathogen, more commonly contracted in the ICU. Enterobacter are ubiquitous in nature. Animals contain Enterobacter in their intestinal tracts allowing for further transmission of the bacteria in soil, water and sewage (Britannica). In…

unknown bacterium, we had to repeat the same step for the citrate agar and nitrate broth. We inoculated the loop, took a small amount of our unknown bacterium, and mixed it in our two tubes containing the citrate agar and the nitrate broth and waited until next lab to continue the process. When we came back next week, we then added two drops of reagent in the nitrate broth to see if the color would change. After doing every experiment, it appeared that the nitrate color was red and the citric…

Enterobacter spp. Enterobacter spp are part of the enterobacteriaceae family. They are facultative anaerobic gram negative bacilli. Their means of motility is by means of petrichous flagella. They produce acid upon glucose fermentation and are methyl red negative, voges-proskauer positive. Optimal growth temperature is 30*C .80% are encapsulated. E. spp have been associated with nosocomial outbreak and are opportunistic pathogens. They can cause numerous infections such as cerebral abscess,…

disease if the host immune system is weakened or the endogenous microbiota altered (12). Consequently, it is essential to be able to identify the organisms because strains are often resistant to the newest antibiotic treatments (11,12). Antibiotic resistance can occur in any species of bacteria. Several other important members of the Enterobacteriaceae including; Citrobacter freundii, Enterobacter cloacae, Providencia stuartii, and Escherichia coli have a large number of resistant strains…

samples were collected and cultivated. The identification of the isolates were performed by using VITEK-2 automated system. Antibiotic susceptibilities were determined by the disc diffusion method according to The Clinical and Laboratory Standards Institute criteria. Results: From a total of 359 patients, who were required invasive ventilation for longer than 48 hours and hospitalised in the ICU, VAP was determined in 16 cases. VUR was 65%, VAP ratio was 4.5% and the rate of VAP was 3.3 per…

wide range auxiliary simple of thiamphenicol with substitution of a flourine iota rather than hydroxylgroup. The antibacterial range of action of florfenicol is more prominent than that of thiamphenicol.[2] It is a fluorinated subordinate of thiamphenicol. A noteworthy system of bacterial resistance advancement to both chloramphenicol and thiamphenicol includes the nearness of chloramphenicol acetyl-transferase (CAT) in resistant organisms. The basic alteration in the particle of florfenicol,…

Unknown Bacteria 228 Adisney Pino Professor Dowding Miami Dade College Section T/R 7:05PM Introduction Enterobacter aerogenes is rod-shaped bacteria that belongs in the family of Enterobacteriaceae. This rod-shaped bacterium is gram negative which makes it a facultative anaerobe which grew perfectly in 37-degree temperature. This species was motile and therefore it can synthesize an enzyme known as ornithine decarboxylase. This bacterium is prevalent in the intestines of…

Biochemical Unknown I. Introduction Cultural Characteristics or morphology and biochemical tests can be used to identify and classify microorganism. By culturing microorganism in nutrient broth, slants, and on nutrient agar plates, the cultural characteristics or morphology can be determined. In this lab, the test tube 2 was incubated in a nutrient broth. The pigmentation of the tube was yellow. The media for test tube 2 was turbid. There were a few sediment on the bottom of the broth. In…