This size difference is suggested to be a consequence of the isoenzymes having different amino acids in the binding pocket of the active site. Histidine-200, histidine-67, and valine-62 in the active site of CA-I appeared to be the main amino acids which could potentially sterically hinder PTS. Further investigations, revealed that histidine-200 of CA-I sterically clashes with PTS, and this may significantly decrease the…
1. If the molarity of the NaOH solution is increased, it would take less drops to observe a colour change. A higher concentration lowers the amount of solution required to produce a reaction due to the presence of more molecules and a higher frequency rate, therefore less of the substance is needed to cause an equilibrium shift. 2. When you add 6.0 M NaOH into the iron (III) thiocyanate ion equilibrium system, the concentration of Fe+3 ion decreases. This causes the equilibrium to favour the…
As you can see, the data recovered from this experiment is very unusual. This is the interpretation and analysis the experiment regarding the cleaning power of the chlorine bleach produced by Clorox and laundry detergent produced by Oxiclean. This interpretation is essential to understand the data and how effective each of the independent variables in this experiment are. As an analysis, there were many key points that made this data relevant. Regarding the coffee, both of the cleaners did quite…
Purpose: The purpose of this experiment was to illustrate how to determine the density of a liquid and regulate volumetric glassware properly. To determine the mass of liquids using a scale, the density of water and (unknown) liquid D were weighed, inside of a volumetric flask. Once the mass was determined in grams, the density was easy to determine, by using the density equation. The second portion of this experiment was to calibrate a 10mL pipette. Once the density was determined for this…
Hair is made up of different layers and structures. It is the tough protein keratin that forms the strength of your hair, nails and the outer layer of skin. Keratin is a large molecule made up of smaller units called amino acids, which join to form a chain. The hair shaft is formed of three layers the medulla, cortex, and cuticle. The medulla is the deepest layer of the hair shaft, only seen in large and thick hairs. The cortex is the middle layer of the hair shaft which provides the strength,…
appear as a bright pink color. In the phenylalanine deaminase test, phenylalanine agar was poured into a small plate and a streak was done after it had solidified. This test is used to detect amino acid breakdown and will use the energy from deaminating amino acids. Phenylalanine becomes phenylpyruvic acid and is detected by acetic iron chloride, which turned the plate green when added. If the bacteria have the phenylalanine deaminase enzyme, the bacteria will become green and considered a…
With the acid, HCl, the pH of the tap water went from 7 to 1.5 after 30 drops, indicating that the concentration of hydrogen ions increased significantly. With the base, NaOH, the pH of the tap water changed from 7 to 12 after 30 drops, indicating that the concentration of of hydrogen ions decreased significantly. These changes occurred in the tap water because there are no chemical buffers present in water. In the potato homogenate, when 30 drops of HCl were added the pH changed from 6 to 3.5…
Formation of alcohol from acid catalyzed addition of water to a carbon-carbon double bond is hydration of alkenes. In this experiment norbornene was used as an alkene to lead the formation of the alcohol. The purpose of this experiment was to make and receive weight of norborneol, percent yield and the melting point of the product. Hydration and the opposite reaction of dehydration establish equilibrium between the two competing processes. To complete the reaction of hydration, double bond will…
pathogens and microorganisms may be present in pools that aren’t treated properly and can result in harmful side effects like swimmers ear or diarrhoea. Disinfectants are used to treat pools and chlorine is by far the most commonly used. Hypochlorous acid (HOCl) is the substance that sanitises these swimming pools (De La Matter, 2012). If kept within specified pH parameters then the system works well however there are many stressors of the backyard pool system. This includes variations in pH…
Introduction: Within this task we were required to determine the ecological state of the Reddam House wetland, which I presume is at a good standard. I decided to test the ecological status of the wetland by gathering samples from the wetland and testing it for various aspects in the water and the plants that are found within the area. Method: • Collected water in a sterilized bottle at Reddam House wetland as well as observed surrounding fauna and flora. • Took the water to Rand Water to have…