Testing Used For Identify Unknown Pathogen Essay

1119 Words May 2nd, 2016 null Page
Section 1: Tests used to identify unknown pathogen
Mario F. Pedero a 30-year-old male visited his physician complaining of painful bumps and soars on his leg. The patient was diagnosed with HIV + 5 years ago. Due to his diagnosis, cutaneous disorders may manifest from the condition itself, or it can lead to opportunistic infections or infections that are more extensive than normal.
The patient was sent to the emergency room were the physical examination revealed skin redness, papule lesions, and central ulcerations present on his left leg. A fever of 38.5 and general feeling of malaise. Systemic symptoms were not present at this time but blood cultures were collected anyway. Based on Mr. Pedero’s medical history a series of tests will be ordered in an attempt to determine the cause of Mr. Pedero’s infection. A swab of the lesion was collected along with drainage from the ulceration and sent to the microbiology lab for culture. A trypticase soy agar (TSA) plate was inoculated and was streaked for isolation then incubated at 37°C for 7 days. The unknown bacterium was off white in color it had an opaque dull optical characteristic. It was round in shape with a colony margin that was entire with smooth edges and the elevation was raised. A MacConkey agar plate was also inoculated and incubated at 37°C for 7 days. The MacConkey agar is selective for the growth of gram negative bacteria, and it differentiates between lactose fermentation and non-lactose fermentation. The unknown…

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