Pseudomonas aeruginosa

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    Pseudomonas Aeruginosa also known as P. aeruginosa is a potential and commonly lethal cause of gram-negative infections (McCarthy, 2015). Infections resulting from P. aeruginosa are related with high rate of mortality and morbidity (Mizuta et al., 2006). Regrettably, the rise in P. aeruginosa resistance increasingly complicates decisions concerning treatment and leads to possible delays in suitable empirical dual antimicrobial therapy (Mizuta et al., 2006). However, early empirical dual antimicrobial therapy is one possible option that can be used that can lead to one selected agent that can be active against a pathogen that is causing infection (Tamma, Cosgrove & Maragakis, 2012). Dual antimicrobial therapy is normally utilized when infection…

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    organism can be identified as Pseudomonas aeruginosa (P. aeruginosa). This organism is an opportunistic pathogen that can infect non-mammalian, plants, and humans. P. aeruginosa belongs to the bacterial family Pseudomonadaceae, and is a gram-negative rod that approximately measures 0.5 to 0.8 µm by 1.5 to 3.0 µm. It can normally be found in water and soil. Its single polar flagellum makes it motile, and it is known to be one of the fastest swimming bacteria found in hay infusion and pond water…

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    Pseudomonas Aeruginosa

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    DISCUSSION: Pseudomonas aeruginosa was selected to study their antimicrobial activity due to the production of pyocyanin, an antimicrobial phenazine pigment. For this study different clinical specimen were collected and from these P. aeruginosa was isolated. To isolate good pyocyanin producing P. aeruginosa, repeated plating of all the isolates on cetrimide agar helped in final selection of the 5 isolates. To examine the antimicrobial activity of those isolates, in vitro inhibition by cross…

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    Plasmid Interference in Regulation of the Type III Secretion System in Pseudomonas aeruginosa Pseudomonas aeruginosa, an opportunistic pathogen, commonly colonizes patients with cystic fibrosis (1) and contributes to a sizeable proportion of antibiotic-resistant nosocomial infections (2). Acute P. aeruginosa infections are characterized by the expression of the type III secretion system (T3SS) comprised of a needle-like apparatus used to inject exoenzymes into neighboring cells (1, 3). These…

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    Gram Negative Bacteria

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    The purpose of the unknown experiment is to understand and identify a gram positive and a gram negative. Bacteria can be very helpful in clinical uses and many pharmaceutical drugs are based on the organism. Knowing how a bacterium works and how it is structured can be crucial to determine how it affects each individual. Several biochemical tests were performing to separate and determine the identities of the microorganisms. Broth tube number 31 containing unknown microorganisms. One of the…

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    TLC Semi Quantitative Dpph

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    [(Absorbance of control – Absorbance of test sample)/Absorbance Control] x100. 2.6. Antimicrobial activity 2.6.1. Microbial cultures ATCC strains of microorganisms for testing the antimicrobial activity were obtained from Department of Microbiology, School of Allied Medical Sciences, University of Botswana, Botswana. The microorganisms included Gram positive cocci : Staphylococcus aureus(ATCC 25923), Methicillin resistant Staphylococcus aureus(ATCC 43300), Staphylococcus aureus from a patient…

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    Officinalis In Plants

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    growth in space and on Earth found that the plants in space were confused when it came to growing in zero gravity, so the steams grew in circles. The plants that were grown low light conditions in space did not have the green color normal plants had, however, the plants that were exposed to light in space were able to show green pigmentation in their roots (Dunbar, 2016). Even though plant growth is minimally affected by the absence of gravity that doesn’t mean that bacterial growth won’t be…

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    How Carbon Sources Affect Biofilm Growth Yeast is single celled and eukaryotic. Biofilms of bacteria can adhere to a device and allow them to withstand environmental changes, which prevents antibiotics from penetrating the cells to kill them. When shifting from a planktonic state to a biofilm, the cells change its proteins. Biofilms produce FLO genes, which code for glycoproteins, cause the yeast to stick together. This is what helps the biofilm form. Once it is forms, the species within the…

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    Introduction With patient safety being at the forefront of hospitals’ priority, it’s imperative for administration to implement infection control protocols. The practice of preventive measures along with staff education is necessary in the reduction of hospital liability, extended inpatient stays and loss in revenue. Although some strategies are standard throughout hospital organizations, data and con-stant monitoring of these procedures will provide insight into compliant areas or those that…

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    Mario F Pedero Case Study

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    Section 1: Tests used to identify unknown pathogen Mario F. Pedero a 30-year-old male visited his physician complaining of painful bumps and soars on his leg. The patient was diagnosed with HIV + 5 years ago. Due to his diagnosis, cutaneous disorders may manifest from the condition itself, or it can lead to opportunistic infections or infections that are more extensive than normal. The patient was sent to the emergency room were the physical examination revealed skin redness, papule lesions, and…

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