The hypothesis for the experiment is that if the temperature and pH increases (but not past optimal levels), and the higher concentration of amylase, then it will result in faster rates of enzyme activity. Therefore, if the opposite were to occur, then the reaction will slow down and not function properly. For concentration, my hypothesis was proved to be correct. The undiluted solution had higher frequencies of collision at the active site of the enzyme and substrate. Which means the percent of amylose as well as absorbance level was higher compared to the diluted samples. The result on the effect of pH on enzyme activity prove my hypothesis to be correct. As the pH increased and each minute, the absorbance levels of percent amylose began
