Sop Standard Operating Procedure

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What is SOP?? A standard operating procedure, or SOP, is a set of step-by-step instructions compiled by an organization to help workers carry out complex routine operations. SOPs aim to achieve efficiency, quality output and uniformity of performance, while reducing miscommunication and failure to comply with industry regulations.

1.SCOPE
This Standard Operating Procedure (SOP) applies to staff and students using the Bench-Top pH Meter in the laboratories.

2.OBJECTIVE
To describe the procedure for installation, operation, calibration, maintenance and troubleshooting of the Bench-Top pH Meter.

3.DEFINATIONS
(a). ↑ oC and ↓ oC Keys: To set the temperature for Manual Temperature
Compensation.
(b). Automatic Temperature Compensation
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- Press the On/Off Key to switch on the meter.
- Rinse the pH electrode with some pH 7.01 buffer solution.
- Immerse the pH electrode into the pH 7.01 buffer solution and stir gently.
- Ensure that the pH electrode is kept submerged approximately 4cm into the solution.
- Ensure that the temperature probe is located as close to the pH electrode as possible.
- Press the CAL Key.
- Wait until the pH symbol, next to the pH buffer value, stops flashing to indicate that reading is stable.
- Press the CON Key to confirm the calibration.
- If [E 4] is flashing, ensure that the correct buffer solution is being used.
- Wait until [E 5] is displayed to indicate that the electrode is still in the pH 7.01 buffer, but expecting a pH 4.01 or 10.01 buffer.
- Rinse the pH electrode with distilled water followed by the second buffer solution to be used.
- Immerse the pH electrode into the second buffer solution (pH 4.01 or pH 10.01) and stir gently.
- Wait until the pH symbol, next to the pH buffer value, stops flashing to indicate that the reading is
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- Inspect the pH electrode for any cracks in its stem or bulb.
- Rinse off any salt desposits with water.
- Soak the pH electrode in General Cleaning Solution for approximately ½ hour to perform general cleaning.
- Soak the pH electrode in Protein Cleaning Solution for 15 minutes if protein deposits are present on the membrane and/or junction of the electrode.
- Soak the pH electrode in Inorganic Cleaning Solution for 15 minutes if inorganic deposits are present on the membrane and/or junction of the electrode.
- Rinse the pH electrode with Oil and Fat Cleaning Solution if oil films or deposits are present on the membrane and/or junction of the electrode.
- Rinse the pH electrode thoroughly with distilled water after performing any of these cleaning procedures.
- Refill the reference chamber with fresh electrolyte and soak it in Storage Solution for at least 1 hour before taking any measurements.

8.PRECAUTION

- Always keep the pH electrode wet and rinse it thoroughly with the sample to be measured before use.
- In order to measure the pH accurately, the temperature factor must always be taken into

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