Biology Lactase Lab Report Example Essay
MATERIALS & METHODS:
Absorbance levels of a 40% ONP concentration in 5 mL were measured at wavelengths ranging from 400-600 nm by 20 nm intervals to find the optimal wavelength for testing. Absorbance levels of a pH 5 buffer and ONP solution were measured at amounts of ONP ranging from 0 to .40 μ mol. A blank was made containing 20% lactase enzyme in 5 mL. In a small beaker 15 mL of pH 5 buffer and 6 mL of enzyme were mixed. To each of 5 cuvettes, initially 1 mL of Na2CO3 was added. As soon as 3 mL of ONPG was added to the small beaker, creating a 0.05 μ mole concentration of ONP, a timer was started and 4 mL of solution was added to the cuvette labeled 0. Four mL of solution was transported from the beaker to each of the 4 remaining cuvettes at the appropriate times of 2, 4, 6, and 8 minutes. After the final 8 minute cuvette was filled the absorbance levels of each cuvette were measured at 415 nm. This experiment was completed 3 times and then repeated 3 times again using both a pH 6 and 7 buffer.
Figure 1 shows the changes in the absorbance level as the