Photosynthesis Of Spinach

Improved Essays
In this study, the effects of both varying temperatures and light intensity upon the photosynthetic rate of spinach leaves were observed. Using the ‘spinach immersion’ technique, the rate of photosynthesis under various conditions was quantified by observing the amount of spinach floating on top of a sodium bicarbonate solution. The sodium bicarbonate solution simultaneously supplied the spinach with carbon dioxide for use in photosynthesis, and caused it to sink. When the leaf underwent photosynthesis and produced oxygen gas, the leaf floated to the surface. It was hypothesized that an increase of either temperature or light intensity would lead to an increased rate of photosynthesis, to a degree. By observing trends in the data, it was concluded …show more content…
In this technique, fifteen cutouts of spinach leaves were placed in various conditions, within a sodium bicarbonate solution. However, without alteration to the spinach cutouts, they would float within the solution. But, by using a soap and sodium bicarbonate solution, soap broke down the waxy hydrophobic cuticles of the spinach, allowing the sodium bicarbonate solution to permeate the leaf’s stomata, replacing the incumbent oxygen. Thus, the leaf would no longer float (due to it being denser than the surrounding solution), and would have a source of both carbon dioxide and water for photosynthesis. And, when the leaf undergoes photosynthesis, the oxygen produced would cause it to rise to the surface, allowing an easy way to measure the rate of …show more content…
As explained in the Results section, there is very clearly a correlation between temperature and rate of photosynthesis - being how quickly the leaves underwent photosynthesis, and floated to the top of the solution. The higher temperatures rapidly achieved 100% floatation, while the lower temperatures had very low floatation rates. Consequently, the hypothesis has been supported by the experiment. This can be explained by the differences in the movement of molecules betwixt each scenario. In the hot scenarios, the molecules would be moving around rapidly, causing there to be a much greater chance of enzyme-substrate interaction, as well as allowing the enzyme to carry out its process much more quickly. In the cold scenarios, the molecules would be moving slowly, decreasing the chance of an enzyme-substrate interaction, and slowing down the enzyme’s processes. To extend this experiment, it is suggested to use 5℃ intervals, as well as going from -10℃ (below freezing) to 110℃ (above boiling). This would allow you to more accurately see the disparity between the hot and cold scenarios, and observe the effects of high temperature on photosynthetic and enzyme

Related Documents

  • Improved Essays

    The main 3 factors that affect it are light intensity, carbon dioxide concentration and temperature. At a short light intensity, as light increases, the rate of the light supported reaction becomes more likely, so consequently photosynthesis generally, increases consistently as shown in figure 2 (Rsc.org P.1). The added photons of light reach the leaf; the more likely of chlorophyll particles that are ionized and the more ATP (the high energy molecule that supplies the energy the plant needs) and NADPH are made. A massive increase in the carbon dioxide concentration increases the rate at which carbon is combined into carbohydrate in the light neutral reaction as shown in figure 3 (Rsc.org, P.2), therefore the rate of photosynthesis commonly increases until limited by another factor that affects photosynthesis. The light neutral reactions of photosynthesis are reliant on temperature.…

    • 1008 Words
    • 5 Pages
    Improved Essays
  • Superior Essays

    Discussion As light intensity increased, so did the measured rate of oxygen consumption and photosynthesis in S. oleracea, in a linear fashion until a plateau was reached. This is due to the fact that the rate of photosynthesis is dependent on light, and if the light intensity is not increased, then the rate of photosynthesis cannot increase. Before CO2 was added to the reaction, photosynthesis occurred at a slower rate because carbon dioxide is a reactant in the photosynthesis equation and by adding CO2, this increases the reactant available to produce glucose and oxygen. With a low supply of CO2, this reaction occurs much more…

    • 1062 Words
    • 4 Pages
    Superior Essays
  • Improved Essays

    Cumnox Reaction Lab Report

    • 1018 Words
    • 5 Pages

    3.2 Effects of particle size The particle size of the catalyst was highly influenced on the reaction rate, when the particle size of the catalyst was decreased, therefore their surface areas was increased and the ratio of catalyst surface and reactant volume was playing a crucial role for controlling the reaction kinetics. The reaction was takes place on the surface of a substance, increasing the surface area should increases the quantity of the substance that is available to react, and will thus increase the rate of the reaction as well. The size and shape of the CuMnOx catalyst particles was a transaction between the minimize pore diffusion effects in the catalyst particles. The particle size provides information about the reaction behavior…

    • 1018 Words
    • 5 Pages
    Improved Essays
  • Improved Essays

    Metabolism Lab Report

    • 759 Words
    • 4 Pages

    In photosynthesis, the plants use carbon dioxide and light energy and produces glucose and releases oxygen. Carbon dioxide is broken down in the Calvin cycle and is produced into a 3 carbon sugar organic molecule. In step 2, the process is cellular respiration. An animal would eat the organic molecule and break it down and take in oxygen and would produce carbon dioxide and water. The carbon dioxide is produced in the Krebs cycle and is released into the atmosphere in where it can be used for photosynthesis.…

    • 759 Words
    • 4 Pages
    Improved Essays
  • Great Essays

    CO2 Partial Pressure

    • 1624 Words
    • 7 Pages

    At high pH, where FeS formation is favorable, the increase in PH2S will increase sulfide ion and sulfide ion concentration, followed by higher super saturation of FeS. High iron sulfide super saturation enhances precipitation and scale formation. Nesic et al. showed that when PH2S is increased, pH at the surface initially increases due to corrosion and release of Fe2+, and then decreases since precipitation of FeS occurs rapidly. They also concluded that at constant pH, as PH2S increases, FeS precipitation can increase faster than the corrosion…

    • 1624 Words
    • 7 Pages
    Great Essays
  • Improved Essays

    Cell Size Experiment

    • 1506 Words
    • 7 Pages

    What factors determine the rate and direction of osmosis? - Temperature because a higher temperature means a faster rate of reaction. Also, concentration of molecules is a factor. Concentrations of molecules have to move from an area of high concentration to an area with low concentration. Lastly, the distance the molecules are, is another factor.…

    • 1506 Words
    • 7 Pages
    Improved Essays
  • Improved Essays

    Rate Of Photosynthesis

    • 1185 Words
    • 5 Pages

    Altering the amount of carbon dioxide given to the plant can also alter the rate at which photosynthesis occurs. Photosynthesis is a process plants undergo that uses water and carbon dioxide gas in the presence of light energy to produce glucose and oxygen gas. This is represented by the formula: 6CO2 + 12 H2O -> C6H12O6 + 6 O2 + 6 H2O. Photosynthesis happens within the chloroplasts of…

    • 1185 Words
    • 5 Pages
    Improved Essays
  • Improved Essays

    It is assumed that and throughout the boundary layer. Physically, increasing both the temperature and the concentration gradients provokes a higher instability at the interface according to reducing the surface tension. This means that increasing both the temperature and the concentration surface tension coefficients and reduces the temperature and the concentration profiles, as shown in Figs.8 and 11. Similar result was obtained experimentally by Aubeterre et al.[26]. This experimental study deduced that the temperature gradient destabilizes the free surface.…

    • 899 Words
    • 4 Pages
    Improved Essays
  • Improved Essays

    The purpose of this lab was to analyze the impact different concentrations of bicarbonate solution had on the rate of photosynthesis in spinach disks. We derived a hypothesis that stated if the concentration of bicarbonate solution increased, the rate of photosynthesis would escalate causing the disks to rise faster to the top of the syringe. When conducting the baseline experiment using a 0.2% bicarbonate solution, the spinach disks starting rising around the ninth or tenth minute. However we realized that the distance we kept between the disks and light source was around 14 cm, 4 cm more than what was required. As a result, we conducted a second baseline experiment with the same concentration of bicarbonate solution measuring the distance…

    • 1056 Words
    • 4 Pages
    Improved Essays
  • Great Essays

    Now, I will discuss my prediction of what will happen during the experiment. Prediction: My prediction is, • As I increase the concentration of hydrochloric acid and add it to the sodium thiosulphate the rate of reaction will decrease. This happens because more particles are being added to the solution when the concentration increases. Therefore, this decreases the rate of reaction and the probability of a collision between reactant particles because there are more of them in the same volume and so increases the chance of a collision forming. This is where the collision theory starts in gases, increase in pressure means those molecules are closer together so there are more…

    • 2652 Words
    • 11 Pages
    Great Essays