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21 Cards in this Set

  • Front
  • Back
What percentage of oxygen does air contain?
20%
Describe the oxygen requirements of obligate or strict aerobes.
can grow with or without oxygen OR are indifferent to oxygen
Describe the oxygen requirements of aerotolerant organisms.
an grow with or without oxygen OR are indifferent to oxygen
Name the 2 genus of bacteria that are classified as strict aerobes.
Bacillus OR Pseudomonas
Name the 2 genus of bacteria that are classified as strict anaerobes.
Clostridium OR Bacteroides
Name one genus of bacteria that are classified as aerotolerant organisms.
Streptococcus
What role does oxygen play in the metabolism carried out by strict aerobes?
final electron acceptor
Name the 2 enzymes that is not present in a strict anaerobe and describe the function of this enzyme.
catalase
superoxide dismutase
enzyme neutralizes by-products of oxygen (free radicals)
When a facultative anaerobe is grown in an atmosphere lacking oxygen, what is one type of metabolism that can occur?
fermentation
What happens when a candle is lighted and placed inside a closed candle jar? Explain.
candle is extinguished (2 pts) because oxygen is consumed by the flame
What enzyme do aerotolerant organisms lack?
catalase
What does “FTM” stand for?
fluid thioglycollate medium
What does “TGYA” stand for?
tryptone glucose yeast extract agar
In a tube of FTM or TGYA, where would you expect to find growth of strict aerobes?
only at the top of the medium
In a tube of FTM or TGYA, where would you expect to find growth of strict anaerobes?
only at the bottom of the medium
In a tube of FTM or TGYA, where would you expect to find growth of facultatives?
throughout the central portion (middle) of the medium
In a tube of FTM or TGYA, where would you expect to find growth of microaerophiles?
under the top surface of the medium (not at the top of the medium)
In FTM, what is the role of sodium thioglycollate?
to reduce the potential for oxidation and reduction
Why is FTM typically pink at the top and colorless in the middle and bottom of the tube? Explain.
The dye in FTM (resazurin) turns pink in the presence of oxygen and there is more oxygen near the top of the medium
Describe the preparation of a TGYA “shake”.
TGYA is inoculated in the liquid state and then shaken to mix the organisms throughout the medium
What is the role of FTM tube?
medium designed to test the aerotolerance of bacteria.