Bios 351: Cultivation Of Anaerobes

Front 1

What percentage of oxygen does air contain?

Back 1

20%

Front 2

Describe the oxygen requirements of obligate or strict aerobes.

Back 2

can grow with or without oxygen OR are indifferent to oxygen

Front 3

Describe the oxygen requirements of aerotolerant organisms.

Back 3

an grow with or without oxygen OR are indifferent to oxygen

Front 4

Name the 2 genus of bacteria that are classified as strict aerobes.

Back 4

Bacillus OR Pseudomonas

Front 5

Name the 2 genus of bacteria that are classified as strict anaerobes.

Back 5

Clostridium OR Bacteroides

Front 6

Name one genus of bacteria that are classified as aerotolerant organisms.

Back 6

Streptococcus

Front 7

What role does oxygen play in the metabolism carried out by strict aerobes?

Back 7

final electron acceptor

Front 8

Name the 2 enzymes that is not present in a strict anaerobe and describe the function of this enzyme.

Back 8

catalase
superoxide dismutase
enzyme neutralizes by-products of oxygen (free radicals)

Front 9

When a facultative anaerobe is grown in an atmosphere lacking oxygen, what is one type of metabolism that can occur?

Back 9

fermentation

Front 10

What happens when a candle is lighted and placed inside a closed candle jar? Explain.

Back 10

candle is extinguished (2 pts) because oxygen is consumed by the flame

Front 11

What enzyme do aerotolerant organisms lack?

Back 11

catalase

Front 12

What does “FTM” stand for?

Back 12

fluid thioglycollate medium

Front 13

What does “TGYA” stand for?

Back 13

tryptone glucose yeast extract agar

Front 14

In a tube of FTM or TGYA, where would you expect to find growth of strict aerobes?

Back 14

only at the top of the medium

Front 15

In a tube of FTM or TGYA, where would you expect to find growth of strict anaerobes?

Back 15

only at the bottom of the medium

Front 16

In a tube of FTM or TGYA, where would you expect to find growth of facultatives?

Back 16

throughout the central portion (middle) of the medium

Front 17

In a tube of FTM or TGYA, where would you expect to find growth of microaerophiles?

Back 17

under the top surface of the medium (not at the top of the medium)

Front 18

In FTM, what is the role of sodium thioglycollate?

Back 18

to reduce the potential for oxidation and reduction

Front 19

Why is FTM typically pink at the top and colorless in the middle and bottom of the tube? Explain.

Back 19

The dye in FTM (resazurin) turns pink in the presence of oxygen and there is more oxygen near the top of the medium

Front 20

Describe the preparation of a TGYA “shake”.

Back 20

TGYA is inoculated in the liquid state and then shaken to mix the organisms throughout the medium

Front 21

What is the role of FTM tube?

Back 21

medium designed to test the aerotolerance of bacteria.