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11 Cards in this Set
- Front
- Back
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Name 2 genus of bacteria that are acid-fast.
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Mycobacterium
Nocardia |
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Name the cell wall substance that makes bacteria acid-fast.
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mycolic acid
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Name the primary (first) stain used in the Ziehl-Neelsen acid-fast stain.
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carbolfuschin
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Name the decolorizing agent used in the Ziehl-Neelsen acid-fast stain.
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acid alcohol
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In a Ziehl-Neelsen acid-fast stain, what must be done while adding the primary stain?
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heat the slide
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In a Ziehl-Neelsen acid-fast stain, what color are the acid-fast bacteria?
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red
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In a Ziehl-Neelsen acid-fast stain, what color are the non-acid-fast bacteria?
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blue
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Name the counterstain used in the Ziehl-Neelsen acid-fast stain.
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methylene blue
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What is the role of heat in acid fast staining?
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it acts as a mordant to make the stain complex more permeable to mycolic acid and cell wall lipids
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What is the Kinyoun acid fast method?
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modification where the concentrations of the primary stain, basic fushsin and phenol are increased, but the bacterial cells are not heated during the staining procedure
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What bacteria are acid fast and non acid fast in this experiment?
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Mycobacterium smegmatis
Staphylococcus aureus |
