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11 Cards in this Set

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  • Back
How are photoautotrophs different from photoheterotrophs?
Photoautotrophs contain autosynthetic pigments such as chlorophyll that convert solar energy to chemical energy by photosynthesis.

Photoheterotrophs derive their energy from photosynthesis but their carbon needs come from growth on organic molecules such as glutamate.
How are photoautotrophs similar to photoheterotrophs?
Both derive their energy from photosynthesis.
What is the name of a solidifying agent used to make solid media?
agar
How is an autotroph different from a heterotroph?
Autotroph derive their carbon requirements from fixating with CO2

Heterotrophs obtain their energy from organic compounds such as polysaccharides.
Why is it best to use distilled water when making microbiological media?
Because tap water contains minerals like calcium, and magnesium which can react with meat extracts and peptone to cause unoculated precipitates.
How is selective media different from differential media?
Selective media has components such as antibiotics that allow only certain types of bacteria to grow.

Differential media has substances that cause some bacteria to take on an appearance that distinguishes them from other bacteria.
If your batch of media has a pH of 4.8 and the pH is supposed to be 6.8, how would you solve this problem?
add NaOH
If your batch of media has a pH of 6.8 and is supposed to have a pH of 4.8, how would you solve this problem?
add HCl
What is the name of the machine that is used to sterilize microbiological media?
autoclave
How does an autoclave kill the microorganisms that may be present in test tubes?
It heats the media at 121 degrees Celsius and at high pressure which kills cells and spores.
What is one reason that microbiological media should be stored in a cool place?
So it doesn’t lose moisture.