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33 Cards in this Set
- Front
- Back
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What effect (increase or decrease) does closing the diaphragm have on resolution?
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decrease
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What effect (increase or decrease) does closing the diaphragm have on image contrast?
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increase
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The objective with a magnification power of 45X is referred to as ____ objective.
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high dry
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The objective with a magnification power of 10X is referred to as the ____ objective?
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low power
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Which three parts of the microscope contain lenses?
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ocular
objectives condenser |
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The objective with a magnification power of 100X is referred to as the ____ objective.
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oil immersion
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The part of the microscope that regulates the amount of light that reaches the slide is the _____.
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diaphragm
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How does using oil immersion enhance the resolving power of the microscope?
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immersion oil prevents the scattering of light rays, allowing more light to reach the slide
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If you are suing a 10X ocular and oil immersion objective, what is the total magnification power of the microscope?
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1000X
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If the total magnification power of your microscope is 50X and you are suing a 10X ocular, what is the power of the objective?
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5X
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Immersion oil must have the same refractive index as ____ to be of any value.
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glass
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What effect (increase or decrease) does closing the diaphragm have on brightness?
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decrease
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What is the resolution of a microscope increased by? 2
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using blue light
raising the condenser to its highest point |
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In general, at what position should the condenser be kept while viewing the slide?
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highest
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What characteristic of a microscope enables one to switch from one objective to another without altering the focus?
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parfocalization
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Substage filters should be of a ____ color to get the maximum resolution of the optical system.
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blue
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TRUE or FALSE
Eyepieces are of such simple construction that almost anyone can safely disassemble them for cleaning. |
False
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TRUE or FALSE
Lenses can be safely cleaned with almost any kind of tissue or cloth. |
False
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TRUE or FALSE
When swinging the oil immersion objective into position after using high-dry, one should increase the distance between the lens and slide to prevent damaging the oil immersion lens. |
False
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TRUE or FALSE
Instead of first using the oil immersion lens, it is best to use one of the lower magnifications first and then swing the oil immersion into position. |
True
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TRUE or FALSE
When the 100X objective is used, the main reason to place oil on the slide is to prevent the objective from cracking the slide. |
False
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When changing from low power to high power, it is generally necessary to
(A) lower the condenser. (B) open the diaphragm. (C) close the diaphragm. (D) Both A and B. (E) Both A and C. |
B
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Microscope lenses may be cleaned with
(A) lens tissue. (B) a soft linen handkerchief. (C) an air syringe. (D) both A and C. (E) A, B and C. |
A & C
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Role of stage adjustment and stage adjustment knob?
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clamping device used for holding and moving the slide arounf on the stage
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How to determine the total magnification of a microscope?
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mutliplying the power of the ocular lens times the power of the objective lens used
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Role of the condenser?
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collects and directs the light from the lamp to the slide being studied
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Does the condenser affect magnifying power?
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no
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Role of diaphragm? 3
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regulates the amount of light that reaches the slide
-improves contrast -reduces the numerical aperature |
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What is the resolving power?
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ability to completely seperate 2 objects
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Resolving power formula?
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d= .5(wavelength) / NA
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What is the limit of resolution?
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.2 micrometers
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What 4 things maximize the resolving power?
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-blue filter because of shorter wavelength
-condenser should be kept at its highest point -diaphragm should not be stopped down too much -immersion oil |
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What is parafocal?
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the image will remain in focus when changing from a lower power lens to higher power.
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