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3 Cards in this Set

  • Front
  • Back
Describe how the preparation of a smear from liquid medium differs from the preparation of a smear from solid medium.
Liquid - use two loopfuls of organisms from broth (2 pts)

Solid - use needle to disperse very small amount of organisms (2 pts)
Why is it necessary to cool a loop or needle completely before inserting it into a bacterial culture?
To prevent splattering which could move bacteria into the air.
When making a smear, why is it necessary to flame the slide after it has dried at room temperature?
To heat-kill and fix the bacteria.