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20 Cards in this Set

  • Front
  • Back

DNA code for traits by dictating

protein synthesis

Proteins are the links between

genotype and phenotype



The molecular chain of command is from

DNA in the nucleus to RNA in nucleus to RNA in the cytoplasm to protein



Transcription

sends out copy of "instructions" in the form of mRNA


mRNA is made by copying DNA strand


(nucleus)

Translation

The mRNA instructions go out into cell


Tell cell what proteins to make (cytoplasm)

Triplet code

the flow of information from gene to protein is based on a......


instructions for the amino acid sequence of a polypeptide chain are written in DNA and RNA as a series of non over lapping, three base "words: called codons.

translation involves switching from

the nucleotide " language" to the amino acid " language"


each amino acid is specified by a codon


most amino acid have more than one possibly codon

genetic code is the

amino acid translation of each nucleotide triplet


three nucleotides each amino acids


sixty- one codons correspond to amino acids


AUG codes for methinine and signals the start of transcription



Redudant

more than one codon for some amino acids



Unambigous

in that any codon for one amino acid does not code for any other amino acid



Translation etc

proteins are strings of amino acids


one gene= one protein


Ribosomes translate


RNA code into proteins



Central dogma of molecular biology

1. Replication- all DNA is copied from other DNA


2. Transcription- all RNA is copied from DNA


3. Translation- all proteins are synthesized from RNA

Transcription three main phases

1. Initiation- attachment of RNA polymerase


2. Elongation- growing the new RNA strand

Transcription 1

RNA polymerase binds to a promoter just upstream from initiation site

Promoter

sequences of 3 nucleotides that tells RNA polymerase that a gene is nearby



more transcription1

RNA polymerase starts moving along DNA, from 5' to 3, adding nucleotides to growing strand of mRNA

more transcription2

keeps going until hits stop sequence/terminator DNA


RNA polymerase and mRNA strand "falls off"

Eukaryotic RNA processioning1



Messenger RNA(mRNA)


Encodes amino acid sequences


conveys genetic messages from DNA to the translation machinery of the cell



Eukaryotic RNA processioning2

In eukaryotes, mRNA must exit the nucleus via nuclear pores to enter the cytoplasm

mRNA processing

processed in several ways before leaving nucleus


Guanine cap added to 5' end


Poly A tail added to 3' end


splicing occurs...removal of introns