Zebra Fish Case Study

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Cancer is a serious cause of death globally. Cancerous cells cannot be distinguished easily from the normal cells. Rapid development of cancerous cells depends on the process of Angiogenesis. Inhibition of the angiogenesis is a novel strategy in cancer treatment. Currently, herbal medicines are also being used, along with chemotherapy to reduce the side effects. For the present study, Neem was selected as it is a versatile tree in India. From the ancient times it has been considered as a mighty tree which gives good health. Roots of that tree were taken to study anti-angiogenesis activity on Zebra fish. The different parts of Neem tree were proven for their cytotoxic activity; hence water soluble fractions of crude methanolic extract of neem roots were taken as a test drug. Fin Regeneration in Zebra fish was selected as an experimental model. Fish were divided into …show more content…
They were kept in a250ml beaker containing 150ml fish water [15] along with drug and observed for lethality for 24hrs. Based on the mortality, dose was increased or decreased by the factor log0.5 or 3.2.
2.2.3 Experiment:
Adult fish were obtained from the local suppliers and kept in large tanks for acclimatization. On the day of experiment, fish were taken out and they were divided into 9 treatment groups: 6 fish in each group. They were anesthetized using 0.1% 2-phenoxyethanol [16]. Their fin was cut up to 50% by using sterilized straight razor blade.
They were again recovered using fish water. Pre and post amputated images were collected and length was measured using scale. 250ml capacity beakers were taken. Upto 150ml filled with fish water which is suspended with the drug, fish were kept in them, one fish per beaker with continuous aeration. Test drug, standard drug (Imatinib) and control were given to each group respectively. Dose for test and standard were determined from the toxicity value [17]. On every alternate day, water was refreshed with the dose up to 15

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