All of the animal experiments were performed under an approved animal use regulation. Wistar rats (200−250 g) were procured from the Laboratory Animal Center. Animals were acclimated for at least 5 days, and fasted overnight before the experiment.
2.4. Standard solutions
Standard solutions of 100.0 µg/mL of FLX, RIS, 9-OH-RIS and OLA each were prepared in methanol. The stock solutions were further diluted with methanol to obtain working solutions for the preparation of calibration standards. All solutions were stored at 4°C and were brought to room temperature before use and could be used for 15 days from the date of preparation. The OLA solution was further diluted with methanol to 12.0µg/ml.
All of the animal experiments were performed under an approved animal use regulation. Wistar rats (200−250 g) were procured from the Laboratory Animal Center. Animals were acclimated for at least 5 days, and fasted overnight before the experiment. …show more content…
Standard solutions
Standard solutions of 100.0 µg/mL of FLX, RIS, 9-OH-RIS and OLA each were prepared in methanol. The stock solutions were further diluted with methanol to obtain working solutions for the preparation of calibration standards. All solutions were stored at 4°C and were brought to room temperature before use and could be used for 15 days from the date of preparation. The OLA solution was further diluted with methanol to
2.4. Standard solutions
Standard solutions of 100.0 µg/mL of FLX, RIS, 9-OH-RIS and OLA each were prepared in methanol. The stock solutions were further diluted with methanol to obtain working solutions for the preparation of calibration standards. All solutions were stored at 4°C and were brought to room temperature before use and could be used for 15 days from the date of preparation. The OLA solution was further diluted with methanol to 12.0µg/ml.
All of the animal experiments were performed under an approved animal use regulation. Wistar rats (200−250 g) were procured from the Laboratory Animal Center. Animals were acclimated for at least 5 days, and fasted overnight before the experiment. …show more content…
Standard solutions
Standard solutions of 100.0 µg/mL of FLX, RIS, 9-OH-RIS and OLA each were prepared in methanol. The stock solutions were further diluted with methanol to obtain working solutions for the preparation of calibration standards. All solutions were stored at 4°C and were brought to room temperature before use and could be used for 15 days from the date of preparation. The OLA solution was further diluted with methanol to