What I Identified Bacillus Cereus Essay

1479 Words May 23rd, 2016 null Page
1. Summary of how I identified Bacillus cereus.
a. First, I performed the gram stain test, I then went on to do the spore stain.
b. The results I obtained from the gram stain were gram positive rods, I could tell they were positive by the purple color to them and the rod shape by the comparison provided in the lab manual (Wilkosz, Peterson). After I confirmed the results I performed the spore stain, which indicated that my sample was positive so it contained endospores. The sample should have shown that there was a red vegetative cell and a green stained endospore (Wilkosz, Peterson), but I must have over rinsed with water because the cells appeared clear with an outline, and the spores that were seen appeared as black dots.
c. The first test performed was the gram stain, the purple color indicated that it was gram positive, this removes all gram negative possibilities including Pseudomonas aeruginosa, Klebsiella pneumonaie, and Proteus vulgaris. Since I also observed that the bacteria were rod shaped, it excluded the cocci specimens Staphylococcus aureus and Streptococcus pyogenes. After performing the spore stain and noticing that there were spores, which concluded that it was Bacillus cereus.
d. I had Unknown plate #12 which I determined contained Bacillus cereus.
2. Anthrax infection caused by Bacillus anthracis.
a. Description of Bacillus anthracis.
i. Bacillus anthracis can enter the body in four different ways: cutaneous, ingestion, injection, and inhalation. The…

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