# Simple Soap Extraction Method

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Research Question:
How does the mass (g) of soap in the Extraction solution affect the mass of DNA extracted (g) from kiwi?

Aim:
To see how the mass of soap in the extraction solution affect the mass of DNA extracted from a kiwi.

Background Research:
DNA is found in the nucleus of the cell. For this experiment, the main goals is to break apart the plant matter, dissolve the membranes of the cell and extracting the DNA found in the nucleus . To extract DNA, we would need a kiwi as DNA exists in all living organisms . The first step is to remove the outer skin of the kiwi before cutting it as it ensures that there will not be any dead DNA in that is useless . As we would need to break the plant matter, mashing the kiwi does just that and
Measured with a digital scale with at least 2 decimal points.
Dependent Variable The mass of DNA extracted from kiwi
(Grams) Use a spatula to extract the DNA from boiling tubes and a beaker on a tarred digital scale to hold and weight it.

Variable Measured Variable Measuring Method Reason for Measuring
Controlled Variables Mass of Salt in the Extraction Solution 0.5 grams of salt is used for each set of extraction solution measured with a digital scale To ensure that the DNA that came out would solidify in the same manner. ml of water in the Extraction Solution 25ml of water is used for each set of extraction solution measured in a measuring beaker by eye To ensure the concentrations of the solutions are equal amongst trials. ml of ethanol used in each boiling tube to precipitate the DNA 15 ml of ethanol is added to each tube, measured in a measuring beaker before poured into the tube. Ensure that the extraction is the same and results are not biased. Temperature of room Air conditioner set at a constant 25°C To ensure that results will not be
Repeat step 12 until all 5 boiling tubes have a layer of 15ml of ethanol. Return the ethanol back to the freezer to preserve temperature for use in the next set.
14. Let the tubes sit in room temperature (25˚C) for 5 minutes. There should be a cloudy clump that forms in the ethanol layer. That is the DNA.
15. Taking the empty beaker that held the strained liquid, put it on the scale and press tare. Taking a boiling tube, slightly tilt it and extract the DNA with the spatula and into the beaker.
16. Repeat step 15 with the rest of the boiling tubes, tare the digital scale in between measurements.
17. Record results in the table below: Mass of DNA extracted (g)
Set Mass of Soap in the extraction solution (g) Trial 1 Trial 2 Trial 3 Trial 4 Trial 5
1 0.5g
2 1g
3 1.5g
4 2g
5 2.5g

18. Repeat steps 10-16 by using the “1”, “1.5”, “2” and “2.5” beakers and record results so 25 sets of raw data is collected. Wash the cheesecloth, spatula, boiling tubes and empty beakers in between performing extraction for each set and blot dry to ensure no residue is left from the previous measurement and will be suitable for