1. Items needed for this experiment are: three nutrient broth tubes, graduated pipet, alcohol, disposable cups, E. coli, S. epidermidis vials, yeast packet, candle, lighter, permanent marker, paper towels, bleach, safety gloves, goggles, face mask and apron.
2. Before beginning the experiment, wash hands with soap and warm water, then put on apron, safety goggles, face mask and gloves.
3. Disinfect the work area with bleach and paper towels. Then pour some bleach in a bucket for anything that will be expose to the microorganisms for disposal.
4. Fill the disposable cup half full with alcohol and put the pipet in it.
5. With the permanent marker, label a nutrient broth tube E. coli, S. epidermidis and S. cerevisiae.
6. Place the tubes that are labeled near the candle on the work surface. Remove the pipet from the cup and expel the alcohol inside the pipet back into the cup by squeezing the bulb and shake out the excess alcohol while squeezing it so no alcohol remains inside. The pipet is now sterile.
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While holding the pipet, pick up one of the labeled nutrient broth and gently remove the cap and gently pass the lip of the broth tube through the flame of the candle to sterilize the surface.
8. Using the Pipet collect .25 mL of broth from the nutrient tube into the culture vial E. coli. Be careful not to touch the sterilized rim of either container with the tip of the pipet or your gloves and place the broth into the vial. Replace the cap and shake the vial well until the table dissolves. Using the pipet, transfer the dissolved ingredients into the labeled nutrient tubes and sterilize the lip of the openings over the flame of the candle before recapping. Repeat steps 6-8 with the S. epidermidis
2. Before beginning the experiment, wash hands with soap and warm water, then put on apron, safety goggles, face mask and gloves.
3. Disinfect the work area with bleach and paper towels. Then pour some bleach in a bucket for anything that will be expose to the microorganisms for disposal.
4. Fill the disposable cup half full with alcohol and put the pipet in it.
5. With the permanent marker, label a nutrient broth tube E. coli, S. epidermidis and S. cerevisiae.
6. Place the tubes that are labeled near the candle on the work surface. Remove the pipet from the cup and expel the alcohol inside the pipet back into the cup by squeezing the bulb and shake out the excess alcohol while squeezing it so no alcohol remains inside. The pipet is now sterile.
7. …show more content…
While holding the pipet, pick up one of the labeled nutrient broth and gently remove the cap and gently pass the lip of the broth tube through the flame of the candle to sterilize the surface.
8. Using the Pipet collect .25 mL of broth from the nutrient tube into the culture vial E. coli. Be careful not to touch the sterilized rim of either container with the tip of the pipet or your gloves and place the broth into the vial. Replace the cap and shake the vial well until the table dissolves. Using the pipet, transfer the dissolved ingredients into the labeled nutrient tubes and sterilize the lip of the openings over the flame of the candle before recapping. Repeat steps 6-8 with the S. epidermidis