Ion Exchange Chromatography Lab

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Lab 1 – Ion Exchange Chromatography
Danielle Small
John Wright
This lab will use ion exchange chromatography – a process in which differently charged ions are separated, by altering the pH of the solution; which shifts the equilibrium of the molecules. During this lab we will separate two compounds from one solute that will be formed by amalgamating a ‘slurry’ – provided by the instructor. Resin or cellulose is used to create a sound support unit in order maintain a constant positive or negative charge respectively, this will allow separation to occur when equilibrium is shifted during the reaction. The column will have a support unit – composed of either cellulose or resin, inserted with varying concentrations of buffer

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