Digestion refers to the heating of the food sample with the presence of sulfuric acid, anhydrous sodium sulfate, and catalyst like copper, selenium, titanium or mercury in a digestion flask. The sulfuric acid is an oxidizing agent that digests the food while anhydrous sodium sulfate speeds up the reaction by raising the boiling point and catalysts to boosts the reaction. In this step, nitrogen (except for those in nitrates and nitrites) are converted into ammonia and other organic matter to C02 and H20. After digestion, neutralization is done. When the digestion is completed the flask is then connected to a receiving flask. Sodium hydroxide is then added to the solution in the flask to form an alkaline which converts the ammonium sulfate into ammonium gas. The ammonia gas is then released from the solution into the receiving flask. The flask still contains excess boric acid. Since the solution in the receiving flask has a low pH, it will convert the ammonia gas into ammonium ion and the boric acid into borate ion. At this point, nitrogen content is estimated by titration. Then after the determination of nitrogen content, it is converted to protein content by the conversion factor. (McClements,
Digestion refers to the heating of the food sample with the presence of sulfuric acid, anhydrous sodium sulfate, and catalyst like copper, selenium, titanium or mercury in a digestion flask. The sulfuric acid is an oxidizing agent that digests the food while anhydrous sodium sulfate speeds up the reaction by raising the boiling point and catalysts to boosts the reaction. In this step, nitrogen (except for those in nitrates and nitrites) are converted into ammonia and other organic matter to C02 and H20. After digestion, neutralization is done. When the digestion is completed the flask is then connected to a receiving flask. Sodium hydroxide is then added to the solution in the flask to form an alkaline which converts the ammonium sulfate into ammonium gas. The ammonia gas is then released from the solution into the receiving flask. The flask still contains excess boric acid. Since the solution in the receiving flask has a low pH, it will convert the ammonia gas into ammonium ion and the boric acid into borate ion. At this point, nitrogen content is estimated by titration. Then after the determination of nitrogen content, it is converted to protein content by the conversion factor. (McClements,