Hydrolysis of Macromolecules Essay
This lab was designed to teach the process of “hydrolysis”, a chemical reaction in which water is added to a polymer, breaking its bonds and forming smaller molecules. A hydrogen cation and a hydroxide anion (which once formed water) break apart and attach themselves to the ends of shorter polymers. Hydrolysis plays an important role in our lives and in the lives of every living thing on earth. Living organisms rely on digestion (hydrolysis) to convert food energy from polymers into monomers, which are easier for our cells to absorb. This study was conducted to show how polysaccharides are broken down by organisms to absorb nutrients through hydrolysis. We used different methods to …show more content…
Biological hydrolysis of polysaccharides by saliva:(BHPS)
In this experiment we saw the effect of saliva in the hydrolysis of polysaccharides. First we collected saliva in a 5-10 ml beaker. Then we prepared a 10 ml of a 1% starch solution, which was made by adding 5 ml of 2% starch and combining it with 5 ml of DI water. Four test tubes, were labeled for the experiment. 3 ml of DI water was added to test tube # 1, 3 ml of 1% starch solution to test tube #2, 3 ml of 1% starch solution to test tube # 3, and 3 ml of 1% glucose solution to test tube #4. On a spot plate 3 drops from each of the test tubes was tested separately with IKI solution, and the results were recorded. The IKI test was also conducted on 1 drop of saliva and the results were recorded on the “before treatment" table. A boiling water bath was prepared, five drops from each test tubes (#1-4) were added to 4 new test tubes along with 10 drops of Benedict’s reagent in each. Then the test tubes were boiled for 1-2 minutes. The Benedict test was also conducted on 5 drops of saliva. The results were recorded on the “before treatment” table.
1 ml of saliva were added to each of our original test tubes #1, #3 and #4, with the exception of #2, and they were mixed well. The test tubes were incubated in a 37 degree Celsius water bath for 15 minutes. Then the type of