The blood sample used was initially centrifuged. This serves dual purpose:
1. To separate WBC from RBC to get faster results, since mature RBC lacks a nucleus, WBC is the primary source of DNA in blood.
2. The centrifugation at 200rpm helps in breaking open the cellular membrane and the cytoplasmic contents are exposed.
The next step involved the breaking down and the emulsification of the fat and proteins that make up the cell's membrane. This is achieved by the addition of both salt and detergent solutions.
Following this, the DNA is separated from the liquid solution by the addition of isopropanol and a second centrifugation. This provides the purified DNA in the supernatant layer as a white precipitate. This can be separated by using a
1. To separate WBC from RBC to get faster results, since mature RBC lacks a nucleus, WBC is the primary source of DNA in blood.
2. The centrifugation at 200rpm helps in breaking open the cellular membrane and the cytoplasmic contents are exposed.
The next step involved the breaking down and the emulsification of the fat and proteins that make up the cell's membrane. This is achieved by the addition of both salt and detergent solutions.
Following this, the DNA is separated from the liquid solution by the addition of isopropanol and a second centrifugation. This provides the purified DNA in the supernatant layer as a white precipitate. This can be separated by using a