Difference Between Concentration Of Ccl And Csr

Decent Essays
The concentration of CDCL and CSER is obtained by solving equation (1) and (2)
CX = A1 / ax1 ………………..... (1)
CY = [A2 – (ax2 × CX)] / ay2…...(2)
Where,
 A1 and A2are absorbance of mixture at λ1 and λ2respectively.
 ax1 and ax2 are absorptivities of DCL at λ1 and λ2, respectively,
 ay1 and ay2 are absorptivities of SER at λ1 and λ2, respectively,
 CXandCYare concentrations of DCL and SER, respectively.

Concentration of DCL and SER in the powder mixture is found by using equation (3) and (4).
CDCL = A1/ 20.14 ………………………… (3)
CSER = [A2 – (43.08×CDCL)]/14.28 … (4)
Where,
 20.14 and 43.08 are absorptivities of DCL at λ1 and λ2 respectively.
 0.00 and 14.28 are absorptivities of SER at λ1 and λ2 respectively.
…show more content…
No. Amount Present in µg/ml Amount Found in µg/ml Amount Found in % SER DCL SER DCL SER DCL
1. 10 50 O9.90 50.00 99.00 100.00
2. 10 50 09.87 50.20 98.70 100.40
3. 10 50 10.00 49.90 100.00 101.80
4. 10 50 09.90 50.20 99.00 100.40
5. 10 50 10.10 50.00 101.00 100.00
6. 10 50 09.87 50.10 98.70 100.20

Table 3: Statistical Validation of Pure Drugs

Drug Mean*
(%) Standard Deviation* Co-efficient of Variation* (% R.S.D.) Standard Error*
SER 99.40 0.9186 0.9241 0.3773
DCL 100.46 0.6772 0.6740 0.2752
SER is Serratiopeptidase and DCL is Diclofenac potassium,* Here Mean is the average of (n=6) results.

Table 4: Data forAnalysis of Tablet
Sr.
No. Label Claim (mg/tab) Amount Found (mg/tab) % of Label Claim SER DCL SER DCL SER DCL
1. 10 50 O9.70 49.40 97.00 98.80
2. 10 50 09.87 50.20 98.70

Related Documents

  • Improved Essays

    Raju Chandra et al; A reliable and reproducible reversed-phase high performance liquid chromatography (RP-HPLC) was developed for the quantitative determination of quetiapine fumarate from marketed bulk tablets. The active ingredient of quetiapine fumarate separation achieved with C18 column using the methanol water mobile phase in the ratio of 30:70 (v/v). The active ingredient of the drug content quantify with UV detector at 359 nm. The retention time of quetiapine fumarate is 5.27 min. A good linearity relation (r2=0.999) was obtained between drug concentration and average peak areas.…

    • 1867 Words
    • 8 Pages
    Improved Essays
  • Decent Essays

    Hydrazine carbamate Hydrazone carbamate LOQ LOD LOQ LOD µg/mL 0.0037 0.0018 0.0037 0.00092 ppm or µg/g 0.366 0.183 0.367 0.092 Injection No. Area counts 1 2053 1255 2005…

    • 952 Words
    • 4 Pages
    Decent Essays
  • Great Essays

    Acetonitrile Case Study

    • 3192 Words
    • 13 Pages

    3. EXPERIMENTAL WORK 3.1 Materials andMethods 3.1.1 Chemicalsand standards used Table.No.15 List of chemicalsand standards used S.No. Chemicals Manufacturer Name Grade 1 Water Merck HPLC grade 2 Acetonitrile Merck HPLC grade 3 0. 22µ Nylon filter Advanced lab HPLC grade 4 0.45µ filter paper Millipore HPLC grade 5 Theo asthalin Cipla syrup form 6 Salbutamol sulphate and theophylline In - House In- House 3.1.2 Instruments used Table.No.16. List of instruments used S.No Instrument name Model number Soft ware Manufacturers Name 1 HPLC-auto sampler –UV detector Separation module2695, UV.detector2487 Empower-software version-2 Waters 2 U.V double beam spectrometer UV 3000+ U.V win soft ware Lab India 3 Digital weighing balance(sensitivity…

    • 3192 Words
    • 13 Pages
    Great Essays
  • Great Essays

    Thin Layer Chromatography

    • 1134 Words
    • 5 Pages

    The Rf value was found in Figure 1 using the equation shown in the data section. It showed that dot “A” had an Rf value of 0.646 and dot “B” had an Rf value of 0.146. The mixture used in the first TLC run contained benzoic acid and biphenyl in the dichloromethane. When the benzoic acid (organic acid) and biphenyl (neutral) was mixed with the 3M NaOH (aqueous solvent), the pH strip indicated that the solvent was base. The 3M NaOH reacted with only the benzoic acid, causing it to produce sodium benzoate while the biphenyl remained the same.…

    • 1134 Words
    • 5 Pages
    Great Essays
  • Superior Essays

    Copper Sulfate Lab

    • 2021 Words
    • 9 Pages

    Mass of starch / Mass of Mixture #6 x 100% = Percent of starch 2.96g / 38.59g x 100% = 7.67% The percent of starch in the mixture is 7.67%. Graph: Evaluation: Percent error of the experiment was 4.2% for the mixture, 8.9% error for obtaining the water, 41% error for obtaining the copper sulfate, and 48% error for obtaining the…

    • 2021 Words
    • 9 Pages
    Superior Essays
  • Great Essays

    The molar mass of KHP was 204.22g. Molarity x Volume (L) = Moles of KHP Moles of KHP x Molar Mass of KHP = grams of KHP 0.1 x 0.05 = 0.005 moles of KHP 0.005 x 204.22 = 1.0211 g KHP Dilution of NaOH and HCl To find out how to dilute NaOH and HCl the equation M1V1 = M2V2 is used. For both the M1 and V1 were given as 0.1 and 250 mL respectively. The M2 was given as 3. The equations were set up as follows: g HCL = 0.1 x 250 = 3 x V2 → (0.1 x 250)/3 = V2 = 8.3 g HCl g NaOH = 0.1 x 250 = 3 x V2 → (0.1 x 250)/3 = V2 = 8.3 g NaOH Preparation of Ascorbic Acid Solution The vitamin C tablet was crushed and 0.5 grams of ascorbic acid was mixed with 50 mL of deionized water to form the Ascorbic Acid Solution Preparation of NaHCO3 solution The baking soda was measured to 0.5 grams and was mixed with 50 mL of deionized water to form the Sodium Bicarbonate Solution.…

    • 1340 Words
    • 6 Pages
    Great Essays
  • Great Essays

    Caprolactam And Nylon 66

    • 1096 Words
    • 5 Pages

    5.00 06 Permanganate No. (PN) in 1 % aq. Soln) (Seconds) W:43:705 Min.10000 07 Absorbance at 290 nm (50% Soln) W:43:706 Max. 0.05 08 Cyclohexanone oxime content (mg/Kg) W:43:707 Max.10.00 09 Iron Content (mg/Kg) W:43:709 Max.0.50 10 Water insoluble (mg/Kg) W:43:710 Max.10.00 Applications Caprolactam is a base material for manufacture of Nylon-6. Its applications are in the manufacture of • Nylon-6 fibres for textile and tyre cord.…

    • 1096 Words
    • 5 Pages
    Great Essays
  • Improved Essays

    Table 1 Composition of Lansoprazole microparticles Formulation code Amount of drug(mg) Amount of polymer chitosan (mg) Drug polymer Ratio Tween 80 (%w/v) F1 100 100 1:1 1.25 F2 100 200 1:2 1.25 F3 100 300 1:3 1.25 F4 100 400 1:4 1.25 F5 100 500 1:5 1.25 F6 100 600 1:6 1.25 Characterization of Lansoprazole microparticle The microparticle will be characterized for following parameters: Percentage yield: After complete drying, the drug loaded microparticle was collected and weighed accurately [9]. The yield of microparticle was calculated by: Percentage yield =( Total wt.of formulation (g))/( Total wt.of drug +Total wt.of excipient) X100 Surface Morphology [10]: The morphology of the microparticle was determined with the aid of Scanning Electron…

    • 1196 Words
    • 5 Pages
    Improved Essays
  • Improved Essays

    Sodium thiosulphate and hydrochloric acid were added aswell. Product collected by vacuum filtration and recrystallized from 96% ethanol. IR- and H- NMR-spectrum were taken to determine the location of the I+. I had a yield of 0,7 g (2,66mmol, 18,2%). The iodide’s position is opposite of the –OH group.…

    • 960 Words
    • 4 Pages
    Improved Essays
  • Superior Essays

    Tinidazole Essay

    • 2519 Words
    • 11 Pages

    The absorbance was measured at λmax on double beam UV- VIS spectrophotometer (UV-1800) against blank. 4.2.5.3 Preparation of calibration curve of Tinidazole in phosphate buffer of pH 6.8 The stock solution was further diluted with phosphate buffer of pH 6.8. Serial dilutions were carried out to get different concentration 1, 2, 3, 4, 5, 6, 7, 8, 9, 10μg/ml. The absorbance was measured at λmax on double beam UV- VIS spectrophotometer (UV-1800) against blank. 4.2.6 Formation of Tinidazole guar gum solid…

    • 2519 Words
    • 11 Pages
    Superior Essays