Antimicrobial Peptide Lab Report
AMPs usually exhibit their antimicrobial effect using a mechanism that involves disruption, followed by an increase in the permeability of the outer membrane (OM) of the bacterial cell which allows the efflux of essential ions and nutrients. Although the exact process is not very clear, several mechanisms have been proposed to explain the behaviour of AMPs, mainly (1) carpet-like mechanism (2) barrel-stave mechanism and (3) toroidal mechanism. 
In the carpet-like mechanism, the AMPs initially bind to the surface of the target cell membrane and cover it partially or …show more content…
Most antimicrobial peptides contain well-defined cationic domains and display a net positive charge ranging from +2 to +9. Cationicity is important as it influences the degree of electrostatic attraction of antimicrobial peptides towards negatively charged phospholipid membranes of bacteria and other microorganisms. Consequently, the polycationic AMPs are selectively attracted to the negatively charged bacterial targets but not to the mammalian cells, which is the very first step in their mechanism of action. This mutual electron affinity is responsible for selective antimicrobial targeting relative to host tissues.