Inhibitory Effect Lab Report

Sample FW13 was exhibited a marvellous antibacterial activity against MRSA at most concentrations during several periods of time. Despite the fact that the inhibitory effect was mild against E. coli and minimum against P. aeruginosa at the maximum concentrations. The gram-positive bacteria was showed an increasing in cell death across various concentrations from low to high. ANOVA was conducted and revealed a significant inhibitory effect for MRSA (P=0.000) at 24 hours. Most concentrations were displayed significant difference in the killing effect at 60 minutes range amongst (P=0.000-0.009) except 800 µg/mL which does not have an inhibitory effect. At the same time, the control was exhibited a significant value (P=0.047) which CFUs was (1.33x109). …show more content…
While the minimum concentration 100 µg/mL (P=0.038) was exhibited the inhibitory effect at 1440 minutes which CFUs counted (1.03x108). Although concentration 400 µg/mL at 24 hours was showed almost near significant value (P=0.052) which was CFUs (1.03x108) and there was a probability to cause more effect if the incubation time was extended. Figure.6 indicates the antibacterial activity of FW13 against P. aeruginosa since this bacteria usually expressed highly resistance. A significant difference via using Kruskal-Wallis test was recorded at the highest concentrations 600 µg/mL (P=0.031) and 800 µg/mL (P=0.033) at 1440 minutes which CFUs counted (3.33x108 and 3.67x108), respectively. Whereas the control was showed near significant value (P=0.052) which CFUs was (4.67x108). Similar to control, the concentration 400 µg/mL was reported a significant value at 60 minutes (P=0.046) which CFUs was (5.67x108) before the bacterial cells showed a resistance to the effect of NPs. However, at 24 hours the P. aeruginosa was started to display a response again and reported near significant value (P=0.059) which may become more effective if the incubation time was