Bacteriophage Lab Report

Great Essays
Introduction: Viral infections can affect many things, including bacteria. When bacteria undergo infections from a virus that virus is called a bacteriophage. After a bacteriophage infects a bacteria it can create two different phases, the lytic or lysogenic cycles. During a lytic stage the bacteriophage causes death to the bacteria. When this happens on an agar plate covered in bacteria it creates a clear spot known as a plaque. Each plaque is created by one virus so from the number of plaques present it is possible to govern how many viruses are in a culture. During this lab this Plaque Counting Assay was used to determine how many viruses were in the solution. The lab was to conduct a serial dilution to determine the titer of the bacteriophage …show more content…
For this lab one conversion and three different statistics were used to compare the results of the serial dilution to the original titer of the T2 Coliphage. First, the data had to be slimmed down to the numbers that are considered reliable, which is between thirty and three hundred, and there resulted in four dependable data points. These four data points were required to be converted into PFU/ml so they could be easily compared to the bacteriophages original titer. The average was the first of the statistical tests done and this required the student to add the four PFU/ml data points up and then divide by four. Standard deviation is the next statistic that was required to solve and the students had to pick between sample or population standard deviation. Sample standard deviation was choose because only a sample of the data was used for the statistics instead of the entire data. This statistic is important so the amount of variation between the data points is known (“Standard Deviation”). The equation is used to calculate this data. Once both the sample standard deviation and the average are calculated it is possible to find the last statistic, coefficient of variation. This data set is determined by dividing standard deviation by the mean. It is important to calculate because it shows the dispersion of the data points around the mean (“Coefficient Of Variation (CV)

Related Documents

  • Superior Essays

    Gram Catalase Test Report

    • 1163 Words
    • 5 Pages

    Abstract: The objective of these series of experiments was to identify two unknown bacteria’s. Broth culture #20 was selected and subjected to qualitative tests for identification. Gram stain tests were performed in order to identify which unknown is gram positive and gram negative. Using selective and differential media, like MacConkey agar which allows the growth of gram negative bacteria only that are able to ferment lactose. Also, mannitol agar was used which isolates and detects gram-positive bacteria.…

    • 1163 Words
    • 5 Pages
    Superior Essays
  • Improved Essays

    is suspected as the cause so they will choose the correct testing media (selective agar media such as SS agar plates) to distinguish Salmonella from other potential bacterial pathogens such as E. coli, Shigella strains, Staphylococcus food poisoning, or from toxins like botulism or pesticides. The majority of Salmonella isolates come from the feces of the infected person. Occasionally, Salmonella can be cultured from blood samples. Serovars are identified by serotyping (detecting bacterial proteins by using specific immunological tests). Definitive diagnosis usually requires that the bacteria be isolated and identified by these techniques.…

    • 797 Words
    • 4 Pages
    Improved Essays
  • Superior Essays

    Rosacea Infection

    • 1220 Words
    • 5 Pages

    The normal strain plays a strong role in maintaining healthy skin. However, when there is a pathogenic strain, it can affect the skin tremendously. Harmful strains can induce harmless ones into becoming pathogenic through different portals of entry that are exposed, and in the skin, certain bacteria can also initiate infection. All in all, this outbreak that the patients acquired was diagnosed as a rosacea infection where the toll-like receptors 2 from a pathogenic strain of rosacea was the cause of antibiotic resistance. We used both the classical methodology and molecular methodology to identify the pathogen that caused the…

    • 1220 Words
    • 5 Pages
    Superior Essays
  • Great Essays

    What Is Microbiology?

    • 1394 Words
    • 6 Pages

    These in retrospect to their names cause bacterial infections, parasitical infections, fungal infections and viral infections. However, although we have studied a vast amount of microbes, for our current scope, let us keen in and focus on a particular type of microbe. These microbes are held responsible for a large amount of illnesses that affect our immune system. Some examples of these illnesses…

    • 1394 Words
    • 6 Pages
    Great Essays
  • Superior Essays

    Microbiota Research Paper

    • 1153 Words
    • 5 Pages

    Microbiota are microscopic organisms such as viruses, bacteria, archaea, and eukaryotes that influence human health through a commensal interaction. Location of microbiota can be internal or external such as the gut, mouth, placenta, skin, and vagina. These microbes are classified into four major phyla: Firmicutes, Bacteriodetes, Actinobacteria, and Proteobacteria. Microorganisms in the Firmicute phylum are typically Gram positive bacteria, either rod-shaped or spherical and function as obligate or facultative anaerobes. Firmicutes are predominately found in the vagina.…

    • 1153 Words
    • 5 Pages
    Superior Essays
  • Superior Essays

    Microorganism Lab Report

    • 1710 Words
    • 7 Pages

    The isolated colonies of the bacteria appeared to have grown and they were approximately 0.2 cm average in length. When I observed their visual appearance they were blurred Wight in color with a transparent slime layer placed on top of the white dotes. After taking notes on the physical appearance of the bacteria’s, a gram stain was conducted on the isolated bacterial sample. The stain process is extremely essential because it separates the bacteria in two groups of gram positive and negatives bacteria’s .in addition, the gram stain shows the color, shape, size, and morphology of the bacteria. In order to narrow our search for unknown microbe, acid fast, spore and capsule stain were…

    • 1710 Words
    • 7 Pages
    Superior Essays
  • Great Essays

    ntroduction The development of the oyster aquaculture become limited as there is a presence of the pathogenic associated microbe that can cause disease which lead to physiological and imbalance in that oyster. There is a few reports that describe the bacterial diversity which is poorly know and required to be further study. (Hernández and Olmos,2006). So, it is important to analyze the bacteria of oyster. This study is carry out to analyze the gram positive bacteria diversity present oyster, Crassostrea iredalei by applying molecular technique like DNA extraction and using of polymerase chain reaction (PCR).…

    • 1716 Words
    • 7 Pages
    Great Essays
  • Improved Essays

    Gram Staining Lab

    • 801 Words
    • 4 Pages

    Information about the Bacteria Pseudomonas aeruginosa During microbiology lab, an unknown bacteria culture in liquid broth was assigned to be identified by conducting a series of various tests. Nearly twenty different tests were conducted on the bacteria, but the most important of these was Gram staining test, gelatin stab test, and oxidase test. The results of these three tests allowed for the determination of the bacteria genus and species. The first test conducted was Gram staining. In the process of Gram staining, a small amount of the cultured bacteria was fixed on a slide with heat.…

    • 801 Words
    • 4 Pages
    Improved Essays
  • Improved Essays

    Cas9 Essay

    • 1113 Words
    • 5 Pages

    CRISPR/Cas9 was originally found in bacteria as five repeating, duplicate sequences next to each other, divided by dissimilar, short sequences of virus DNA. The Cas9 part is a linked nuclease that is aided by a guide RNA, used to cut DNA. The CRISPR/Cas9 system is an immune response in bacteria that works when a virus, releases its genetic code into the bacteria. The virus DNA is stored in the bacteria DNA, so that it can specifically attack that virus, where it to invade again. When the virus attacks the bacteria again, its DNA is detected through an RNA base pairing method.…

    • 1113 Words
    • 5 Pages
    Improved Essays
  • Improved Essays

    E. Coli Bacteria

    • 971 Words
    • 4 Pages

    E. coli can also cause clinical disease in immune compromised patients such as the elderly. The diagnosis of E. coli found in an elderly woman, Doris, makes sense especially with someone who has a history of UTI and kidney infections. Additionally, this species can acquire virulence factors encoded on their plasmids or in bacteriophage DNA, causing some strains to have enhanced virulence. E. coli 0157:H7 is a particularly virulent strain that has been linked with multiple kinds of contaminated food and has caused many deaths². This strain of E. coli forms a toxin that destructs blood vessels and causes very severe…

    • 971 Words
    • 4 Pages
    Improved Essays