Isolating Phage

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This study is about isolating phage. The first step was to do an enrichment isolation then do a plaque assay and spot test. After that the next protocol was do a plaque streak, which was conducted numerous of times. Then serial dilution was conducted and RE Digests of Phage DNA as well. Last step was protocol 10.3, gel electrophoresis. The relevance of this study is important to field of biology because bacterial infections could be treated with bacteriophages. Bacteriophages can be a solution to antibiotic resistance because phages are viruses that are able to infect bacteria. This can tremendously help and set a new pathway in biology. The results were that phage was isolated. The conclusion is that gordonia host didn’t produce results, which …show more content…
The first step is to set up the gel electrophoresis equipment with gel prepared according to the casting Agarose gels protocol. Then set up restriction enzyme digest samples for electrophoresis. Then add 5 µl of concentrated 6x loading dye to each 25 µl restriction enzyme sample. Then load the gel in this order: ladder, Uncut DNA, BamHI, Clal, EcoRI, Haelll, HIndIII, Sall. Next, is to load the gel with the right amount of volume of DNA ladder. Use micropipettor for each sample (pipette 20 µl) of each RE reaction into the wells in order. Then plug the electrodes into the appropriate locations on the power supply. Turn power on and and set voltage to 100 V. Make sure to take a picture of the end result. (Poxleitner et al. …show more content…
When changing hosts from gordonia terrae to M.smeg, the results for other experiments were able to get phage. Adopting from those experiments allowed this experiment to successfully get phage. Due to this, the results proved the experiment hypothesis. Different ways to help produce results is to continue using aseptic technique to make sure there’s no contamination. If the results came out positive for phage this could help future research because scientists are trying to find ways to use phage in antibiotic resistance. Due to the fact that the number of antibiotics are decreasing and that the microorganisms are increasing, phages present a new idea on how to help with this situation. Phages can be an alternative to antibiotics and help with the antibiotic resistance crisis. This could tremendously make a difference and advance in the field to help find ways to combat antibiotic resistance. Future studies that could be done based on the information gained from research is to continue conducting experiments with phage and to further find various phages that are yet to be

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