Isolating Phage

Superior Essays
This study is about isolating phage. The first step was to do an enrichment isolation then do a plaque assay and spot test. After that the next protocol was do a plaque streak, which was conducted numerous of times. Then serial dilution was conducted and RE Digests of Phage DNA as well. Last step was protocol 10.3, gel electrophoresis. The relevance of this study is important to field of biology because bacterial infections could be treated with bacteriophages. Bacteriophages can be a solution to antibiotic resistance because phages are viruses that are able to infect bacteria. This can tremendously help and set a new pathway in biology. The results were that phage was isolated. The conclusion is that gordonia host didn’t produce results, which …show more content…
The first step is to set up the gel electrophoresis equipment with gel prepared according to the casting Agarose gels protocol. Then set up restriction enzyme digest samples for electrophoresis. Then add 5 µl of concentrated 6x loading dye to each 25 µl restriction enzyme sample. Then load the gel in this order: ladder, Uncut DNA, BamHI, Clal, EcoRI, Haelll, HIndIII, Sall. Next, is to load the gel with the right amount of volume of DNA ladder. Use micropipettor for each sample (pipette 20 µl) of each RE reaction into the wells in order. Then plug the electrodes into the appropriate locations on the power supply. Turn power on and and set voltage to 100 V. Make sure to take a picture of the end result. (Poxleitner et al. …show more content…
When changing hosts from gordonia terrae to M.smeg, the results for other experiments were able to get phage. Adopting from those experiments allowed this experiment to successfully get phage. Due to this, the results proved the experiment hypothesis. Different ways to help produce results is to continue using aseptic technique to make sure there’s no contamination. If the results came out positive for phage this could help future research because scientists are trying to find ways to use phage in antibiotic resistance. Due to the fact that the number of antibiotics are decreasing and that the microorganisms are increasing, phages present a new idea on how to help with this situation. Phages can be an alternative to antibiotics and help with the antibiotic resistance crisis. This could tremendously make a difference and advance in the field to help find ways to combat antibiotic resistance. Future studies that could be done based on the information gained from research is to continue conducting experiments with phage and to further find various phages that are yet to be

Related Documents

  • Improved Essays

    The thermocycler is a directed machine to program the alteration of the temperature in the reaction for denaturing and synthesizing. The temperature regulation is essential to optimize the results. The length of the DNA region to be copied depends on the cycle repeats (25 - 40) and time (2 - 4 hours). The new DNA made in one round can serve as a template in the next round of DNA synthesis. The number of DNA molecules double in each cycle due to the multiple copies of primers and Taq polymerase.…

    • 916 Words
    • 4 Pages
    Improved Essays
  • Improved Essays

    Our goal within the plasmid isolation lab was to isolate the bacterial plasmid without any damage to the DNA. The formation of the clear lysate was essential because it allowed us to isolate the bacterial cells from removed media and later expose the DNA. Resuspension solution helped to resuspend the cells, after centrifugation, and prepare the cells for lysis solution. The lysis solution functions to degrade the cell membrane and spill its components. Then the Alkaline Protease functioned as the inhibitor of any DNases and denatured dsDNA to ssDNA shortly, by disturbing H-bonds between bases.…

    • 1208 Words
    • 5 Pages
    Improved Essays
  • Improved Essays

    Measured with a digital scale with at least 2 decimal points. Dependent Variable The mass of DNA extracted from kiwi (Grams) Use a spatula to extract the DNA from boiling tubes and a beaker on a tarred digital scale to hold and weight it. Variable Measured Variable Measuring Method Reason for Measuring Controlled Variables Mass of Salt in the Extraction Solution 0.5 grams of salt is used for each set of extraction solution measured with a digital scale To ensure that the DNA that came out would solidify in the same manner. ml of water in the Extraction Solution 25ml of water is used for each set of extraction solution measured in a measuring beaker by eye To ensure the concentrations of the solutions are equal amongst trials. ml of ethanol used in each boiling tube to precipitate the DNA 15 ml of ethanol is added to each tube, measured in a measuring beaker before poured into the tube.…

    • 1816 Words
    • 8 Pages
    Improved Essays
  • Improved Essays

    Later some Elution Buffer was used to separate the DNA from the membrane of the spin column. Day two consisted of making the plasmid DNA resistant to Kanamycin and Ampicillin. Day three we made 0.8% agarose gel and ran electrophoresis to be able to see the weight of the DNA. Day four we genetically transferred the ligated plasmid DNA to E.coli and plated the cell mixture. Day five we looked at our results and went over them.…

    • 1536 Words
    • 6 Pages
    Improved Essays
  • Decent Essays

    Bacteria Lab Report

    • 343 Words
    • 2 Pages

    The bacteria used in the experiment are Escherichia coli, Pseudomonas aeruginosa, Proteus vulgaris, Klebsiella pneumoniae, and Salmonella pneumonia. These all bacteria will be culture in the nutrient broth. The first stage is making Nutrient Broth. Weigh out 5.8 grams of nutrient broth powder. Dissolve completely the nutrient broth powder in beaker containing 200 ml of distilled or the deionise water.…

    • 343 Words
    • 2 Pages
    Decent Essays
  • Improved Essays

    We also took a startch and a protein (albumin) and made two more solutions. We had a total volume for each protein and starch of 2,100uL in the 5 test tubes of each concentration of protein and starch. We had to add an additional 1,500uL of water to the starch solutionWe used the Biuret’s reagent(2,000uL) for the protein concentration because the reagent detects protein molecules which causes the solution’s color to change. We used the Lugol’s reagent(500uL) for the starch concentrstion because the reagent detects starch molecuels which cause the solution’s color to change. We used the C1V1=C2V2 equation to figure out how much water and stock solution of starch/protein we had to add up to final volume of stock protein/starch solution.…

    • 855 Words
    • 4 Pages
    Improved Essays
  • Improved Essays

    Aligning the wells at the negative end of the box was very important. With the gel being inside the box, running buffer was used to fill up the gel until the wells were covered with the buffer. Next step was to use the micropipette and provided tips to fill up each well with 35 uL of each sample in their separate wells. Everything was checked to make sure everything was properly done. Electrodes were hooked in their desired locations, power source was provided to the gel box, and timer was set for 15 minutes.…

    • 1612 Words
    • 7 Pages
    Improved Essays
  • Great Essays

    This study is carry out to analyze the gram positive bacteria diversity present oyster, Crassostrea iredalei by applying molecular technique like DNA extraction and using of polymerase chain reaction (PCR). This study permit us to identify what and how bacteria that become pathogenic to oyster. The starting point in identification of microorganism…

    • 1716 Words
    • 7 Pages
    Great Essays
  • Improved Essays

    Introduction: In this lab experimentation, the group conducted Deoxyribonucleic acid isolation and restriction analysis on a plasmid from Escherichia coli cells. Plasmids are small circular DNA that are in the bacterium cells. Escherichia coli is a gram- negative bacterium that is known for variable reaction to antibiotics, and can be genetically manipulated. The gram- negative bacterium, Escherichia coli can be genetically manipulated by extracting a certain plasmid that allows it to resist ampicillin. If a bacterium is carrying a plasmid that encode for antibiotic resistance, it will grow and become reproductive in the environment of that antibiotics.…

    • 1324 Words
    • 5 Pages
    Improved Essays
  • Great Essays

    After mixing the agarose and bromide, we constructed the chamber and proceeded to carefully pour the agarose into the chamber. After pouring the agarose, we proceeded to add 1X TBE buffer to the chamber. To load and run Gel 1, we added 6µl of 10X Blue Juice to our PV92 tube. We then obtained a tube of DNA size markers which contained known DNA fragments of 766, 500, 300, 150, and 50 base pairs which were added to Well 2 and measured at 10µl (Leicht and McAllister, 2016) . We then proceeded to fill wells 3-5 with 20µl of our PV92 samples, each teammate getting their own well.…

    • 1491 Words
    • 6 Pages
    Great Essays