Bacterial Transformation By Derek Tang Essay

1911 Words Nov 4th, 2014 8 Pages
BACTERIAL TRANSFORMATION
By Derek Tang
BIO 281 Lab section 80243

Abstract: The objective of this experiment was to observe the results of bacterial transformation in various growth conditions and determine how successful the altered organism is after the transformation. The organism used was E. Coli because singular cell organisms can have a new gene more easily inserted than multi-cellular organisms. The gene which was inserted is pGLO that carried the gene encoding GFP and a gene for resistance to the antibiotic ampicillin. The GFP gene, in the pGLO, is a gene that that produces a fluorescent protein, which glows when it is placed under an ultraviolet light. For the gene to be turned on, the sugar arabinose is added. In order to determine how successful the altered organism became, quantitative data in the amount of colonies were observed in the different agars. In general, the ampicillin would destroy the bacteria if it did not have the gene; however because of the gene the bacteria should have a better chance of surviving the ampicillin. This is showed by the amount of colonies that are seen in the agar when shown under an ultraviolet light. This supported the hypothesized importance of genetic transformation; as it leads to an increase of survival and for the possible chance for that gene to be passed on to other generations.

Introduction: In order to survive, many organisms pass on their genes to their next generation. However there will be cases when…

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