coli is considered a mesophile, meaning it grows at moderate temperatures, between 20°C and 40°C, with its optimal growth temperature being 37°C (Piispanen, 2017, Fall Lecture). The results demonstrates that 37°C is the most optimal temperature for growth, as this plate had the highest number of colonies present. However, to support this statement of E. coli being a mesophile, there would had to have been growth on the plate incubated at 23°C. To clarify these findings further and investigate why there was no growth on the 23°C, the experiment could be performed an additional time. However, the sample could be diluted more, using a dilution other than 10-6, such as 10-8. By using a more diluted sample, the number of colonies may be less plentiful, thus allowing for an easier count of the colonies on each plate or possibly only allowing for growth on the 37°C, as it is suggested to be the most optimal temperature for growth (Piispanen, 2017, Fall Lecture). By decreasing the number of bacterium in the starting dilution, it may allow only the plate incubated at the optimal temperature of 37°C to produce colonies, and restricting the other plates incubated at other
coli is considered a mesophile, meaning it grows at moderate temperatures, between 20°C and 40°C, with its optimal growth temperature being 37°C (Piispanen, 2017, Fall Lecture). The results demonstrates that 37°C is the most optimal temperature for growth, as this plate had the highest number of colonies present. However, to support this statement of E. coli being a mesophile, there would had to have been growth on the plate incubated at 23°C. To clarify these findings further and investigate why there was no growth on the 23°C, the experiment could be performed an additional time. However, the sample could be diluted more, using a dilution other than 10-6, such as 10-8. By using a more diluted sample, the number of colonies may be less plentiful, thus allowing for an easier count of the colonies on each plate or possibly only allowing for growth on the 37°C, as it is suggested to be the most optimal temperature for growth (Piispanen, 2017, Fall Lecture). By decreasing the number of bacterium in the starting dilution, it may allow only the plate incubated at the optimal temperature of 37°C to produce colonies, and restricting the other plates incubated at other