After receiving approval, one student was chosen to handle the materials. While wearing sterile gloves to avoid hand microbes from contaminating the samples, the student obtained two cotton swabs and two agar plates. Each plate was marked and labeled with the group’s information as well as the respective origins of the microbial cultures. In addition, the plate was divided into three equal sections to aid in the steps to follow. After swabbing the respective location of which the sample was to be gathered from, the cotton swab was then gently streaked over the agar on the plate following the marked sections in a zigzag pattern. Special attention was taken to make sure that there was touching endpoints between the first and the second section as well as the second and the third section (Worstl 2017). Then the agar plates were sealed and incubated for one week at twenty-eight degrees Celsius. In this experiment, there was no control value, however, the constant values were the standardized agar plates, the constant time and temperature of incubation, the sterility of the cotton swabs, the time of sample gathering, and the method of streaking. The independent variable was the location from which the samples were gathered, and the dependent variable is the microbial biodiversity, which is measured in richness and
After receiving approval, one student was chosen to handle the materials. While wearing sterile gloves to avoid hand microbes from contaminating the samples, the student obtained two cotton swabs and two agar plates. Each plate was marked and labeled with the group’s information as well as the respective origins of the microbial cultures. In addition, the plate was divided into three equal sections to aid in the steps to follow. After swabbing the respective location of which the sample was to be gathered from, the cotton swab was then gently streaked over the agar on the plate following the marked sections in a zigzag pattern. Special attention was taken to make sure that there was touching endpoints between the first and the second section as well as the second and the third section (Worstl 2017). Then the agar plates were sealed and incubated for one week at twenty-eight degrees Celsius. In this experiment, there was no control value, however, the constant values were the standardized agar plates, the constant time and temperature of incubation, the sterility of the cotton swabs, the time of sample gathering, and the method of streaking. The independent variable was the location from which the samples were gathered, and the dependent variable is the microbial biodiversity, which is measured in richness and