General Unknowns
Bacterium Vitality en Vida
Bacteria play a vital role in our daily lives. It is therefore our right to know the importance of being able to distinguish certain strains of bacteria. With the right knowledge we would be able to come up with a cure for a disease. An identity gives way to knowledge, to better understanding and access to information on how an organism works and how we can prevent its worsening effects or isolate its benefits. Scientists have been trying to discover and study different strands each and every day to keep up with the future’s demands for medicine and cure mainly because we are all susceptible to diseases. People that study these organisms use different techniques to reveal the identity of …show more content…
We were asked to identify an unknown from a broth culture; a mixed culture perhaps. I picked the unknown slant number 5. I have written a dichotomous key to guide which tests I am going to perform. The two general unknowns given by the instructor are expected to be from a gram positive and the other to be gram negative bacteria. One of which will be a cocci and the other a rod shape. The most important part of the study of bacteria is the knowledge of its identity. With the right approach knowing a bacteria’s identity is not as hard at all. Just as long as someone knows what he or she is doing these will go smoothly. First, we have to grow them in differential medium for culture separation. Then after 48 hours of incubation we started to identify the gram stain. From that, different tests were performed to be able to find the specific identity of bacteria further tests will be explained in each specific method …show more content…
In the glucose test, like MSA, this medium contains the pH indicator, phenol red. If an organism ferments glucose, then acidic by products are formed and pH indicator turns yellow. SIM tubes are inoculated with a single stab to the bottom of the tube. If motile, the growth will radiate from the stab mark and make the entire tube appear turbid. The strain of Proteus mirabilis is motile (Microlab). The pH indicator (methyl red) is added to the culture broth and the pH is low a red color will appear indicating a mixed acid fermentation. The differential ingredient in MSA is the sugar mannitol. Organisms capable of using mannitol as a food source will produce acidic by products of fermentation that will lower the pH of the media. The acidity of the media will cause the pH indicator, phenol red, to turn yellow. S. aureus is capable of fermenting mannitol, but not P.
Bacterium Vitality en Vida
Bacteria play a vital role in our daily lives. It is therefore our right to know the importance of being able to distinguish certain strains of bacteria. With the right knowledge we would be able to come up with a cure for a disease. An identity gives way to knowledge, to better understanding and access to information on how an organism works and how we can prevent its worsening effects or isolate its benefits. Scientists have been trying to discover and study different strands each and every day to keep up with the future’s demands for medicine and cure mainly because we are all susceptible to diseases. People that study these organisms use different techniques to reveal the identity of …show more content…
We were asked to identify an unknown from a broth culture; a mixed culture perhaps. I picked the unknown slant number 5. I have written a dichotomous key to guide which tests I am going to perform. The two general unknowns given by the instructor are expected to be from a gram positive and the other to be gram negative bacteria. One of which will be a cocci and the other a rod shape. The most important part of the study of bacteria is the knowledge of its identity. With the right approach knowing a bacteria’s identity is not as hard at all. Just as long as someone knows what he or she is doing these will go smoothly. First, we have to grow them in differential medium for culture separation. Then after 48 hours of incubation we started to identify the gram stain. From that, different tests were performed to be able to find the specific identity of bacteria further tests will be explained in each specific method …show more content…
In the glucose test, like MSA, this medium contains the pH indicator, phenol red. If an organism ferments glucose, then acidic by products are formed and pH indicator turns yellow. SIM tubes are inoculated with a single stab to the bottom of the tube. If motile, the growth will radiate from the stab mark and make the entire tube appear turbid. The strain of Proteus mirabilis is motile (Microlab). The pH indicator (methyl red) is added to the culture broth and the pH is low a red color will appear indicating a mixed acid fermentation. The differential ingredient in MSA is the sugar mannitol. Organisms capable of using mannitol as a food source will produce acidic by products of fermentation that will lower the pH of the media. The acidity of the media will cause the pH indicator, phenol red, to turn yellow. S. aureus is capable of fermenting mannitol, but not P.