Aerobic Respiration Lab Report

Hypothesis: Aerobic Respiration: If a phosphate buffer, dichlorophenol-indophenol and succinate are added to a mitochondrial extract then the succinate molecules will bind to an enzyme complex (succinate dehydrogenase). Because the enzyme and FAD are a part of the same complex, initiating succinate oxidation, this reaction reduces FAD to FADH2. Thus the oxidation of succinate to furmarate. Anaerobic Respiration: If a solution of yeast and glucose is combined and placed in different incubation temperatures, then the amount of gas that is released from the test tube will increase with temperature. This is because the “Kinetic Theory” explains that the extent of a molecules movement depends on its state and temperature. As temperature increases, the particles can escape better and move around colliding together. These collisions cause the reaction. Heat is also a catalyst in all reactions. Fermentation: If a solution of yeast and six different carbohydrate sources are combined and placed in 45C incubators, then the volume of gas expelled from each test tube will increase depending on the carbohydrate. Because yeast is an enzyme, it wants to work at a certain temperature the best, this being 45C. According to the lock and key theory, enzymes are protein molecules and each enzyme has a different shape for a specific reactant to fit into, …show more content…
That was, only until it hit 45C in the incubators, after that the gas volume started to drop the hotter it became in the different incubators. As explained before, the enzymes have a different shape for certain reactants to fit into. When a protein is denatured, this is irreversible and the mechanism can no longer work. When the yeast was at 45C and 60C is when the volume of CO2 produced was at its highest, while ice and boiled yeast were the most ineffective temperatures for the volume of CO2 that was