The Effect Of Chemistry: Lab Analysis Of Yeast Catalase

Register to read the introduction… In this part of the lab, we pour 20 mL of Hydrogen Peroxide and 1 mL of yeast catalase in the reaction chamber and then submerge it in the water bath. After it was done, it can be concluded that the gas levels increased because when we first started timing it, the gas levels were at 76, but as time went by, the gas levels started to increase and in the end of the last interval, the gas level increased all the way up to 100. This result occurred because there were many active sites that were empty for the substrate to bind to and since they binded to the active sites, it started increasing a lot at the beginning. However, when the active sites started getting more occupied, the substrate could not bind to as much active sites, so the gas levels started to increase alittle less as time went on and in the end, there was no active sites that were …show more content…
After we did the experiment for pH 4, it can be concluded that the gas level increased because it started at a gas level of 7 and in the end, it was at 34. After the experiment for pH 7, the gas level increased because it started at a gas level of 9 and in the end, it was at 36. After the experiment for pH 10, the gas level increased even more than pH 7 because the gas level started at 10, but in the end, it was at 40. Even though those are the results we got in the lab that is not how it is supposed to be that because pH 7 was supposed to have the highest increased gas levels because this enzyme works best in a neutral pH environment, so it should be pH7 instead of pH …show more content…
After we did all three of the concentrations, we can conclude that all three of them increased their gas levels. However, 0% NaCl concentration increased the most because since it has no salt, it could work in its normal environment and the gas levels increased a lot and it was nowhere near getting denatured. In the 2% NaCl concentration, the gas levels increased, but not as much as 0% NaCl concentration because salt is known for denaturing enzymes over time, so as time went on the experiment, the enzyme starts getting denatured and you notice it in the end because the gas levels are increasing less and less. In the 10% NaCl concentration, the gas level increased, but not as much as 2% NaCl concentration because there is so much more NaCl, so the enzyme will get denatured even quicker and the gas level will increase less and less early on in the