Hema Blood Collection Ajfb

Front 1

Total Blood volume in an Adult

Back 1

5-6 L or

5-8% Of the Body Wieght

Front 2

% Formed Elements in the blood

Back 2

45%

Hint 2

1. RBCs

2. WBCs

3. Platelets

Front 3

% Fluid Portion in the Blood

Back 3

55%

Hint 3

90% Water

10% Proteins (Albumin, globulin, fibrinogen) CHO, Vit, Hormones, Enzymes, Lipids, and Salts

Serum - liquid portion of clotted blood

Plasma - liquid portion of anticoagulated blood

Front 4

Prevents coagulation by chelating calcium

Most common

For CBC, Plt count

Modified Westergren ESR

Back 4

EDTA/

Versene/Sequestrene

Hint 4

8 Inversions

(8-10)

Front 5

Can't be used for coag studies, interferes w/ Factor V &

Thrombin-Fibrinogen Reaction

Back 5

EDTA

Front 6

Platelet encircling peripheral borders of neutrophils

Due to serum factor that reacts in the presence of EDTA

Back 6

Platelet Satellitosis

Hint 6

Correction:

Use of Na Citrate

Front 7

Excess EDTA Effects:

Back 7

1. Shrink RBCs

2. Degenerative changes in WBC

3. Swell & Break Plt

4. Hgb no effect

Hint 7

1. Dec spun Hct

Inc MCHC

Falsely LOW ESR

3. Falsely Inc Plt Count

Front 8

Changes in Blood @ Room Temp after 3 hrs:

Back 8

1. Degenerative Cellular Changes

2. WBCs - cytoplasm vacuolation, more homogenous nuclei, poorly defined cytoplasmic borders, devpt of irregulary shaped nuclie

3. Plt Inc in Size and Disentegrate

Front 9

Changes in Blood @ room temp after 6 Hrs:

Back 9

1. RBCs Swell, Inc MCV

2. Inc OFT

3. Dec ESR

Front 10

Slow down changes in Blood for upto 24 Hrs

Back 10

Refrigeration @ 4C

Front 11

Prevents coag by binding calcium in the blood in a soluble complex

Helps Plt retain functional capabilities

Sugar Water Test/Sucrose Hemolysis for PNH

Back 11

Sodium Citrate

Light Blue Top

Hint 11

3-4 Inversions

Front 12

Ratio of Anticoag to Blood in Citrate (Light Blue)

Back 12

1:9

Hint 12

1 Part 0.109 M (3.2%) Sodium Citrate: 9 Parts WB

Front 13

Also contain Citrate

Used in original/standard

Westergren ESR

1:4 Ag:Blood

Back 13

Black Top

Front 14

Prevents coag by interacting w/ anti-thrombin III and subsequent inhibition of Thrombin

Natural Anticoag

Used In vivo/vitro

Cardiopulmonary Bypass

Back 14

Heparin

Hint 14

8 Inversions (8-10X)

* May be used in conc of

15-20 or 30 U/mL

Front 15

Effect on Wright's stained blood smear prepared from heparinized blood

Back 15

Blue Background

Front 16

Anticoagulant used in Osmotic Fragility Test (OFT) &

Leukocyte Alkaline Phosphatase (LAP)

Back 16

Heparin

Hint 16

1. Not used in Coag studies, inhibits thrombin

2. May cause error in automated cell counting

Front 17

Acts by forming insoluble complexes w/ calcium

Back 17

Oxalates

Hint 17

8-10 Inversions

Front 18

Double/Balanced Oxalate

Back 18

1. Potassium Oxalate (Paul-Heller) 2 Parts

2. Ammonium Oxalate (Wintrobe) 3 Parts

Hint 18

1. Shrinks Cells

2. Swells

Front 19

Inhibits glycolysis, preserves glucose

Not an Anticoag

Combined w/ K oxalate if anticoag needed

Back 19

Na Fluoride

Hint 19

8 Inversions

Front 20

Blood Culture Collection

Anti-phagocytic

Anti-complement

Back 20

SPS

Yellow Stopper

Front 21

For DNA Testing

HLA Phenotyping

Back 21

Acid Citrate Dextrose (ACD)

Yellow Stopper

Front 22

Veipuncture:

Order of Draw

Back 22

1. Blood Culture Tubes (Yellow)

2. Coagulation (Blue)

3. Serum (Non-additive) Tubes (w/ or w/out clot activator or gel separator)

4. Heparin (Green)

5. EDTA (Lav Top)

6. Sodium Fluoride - Glycolytic Inhibitor (Gray)

Hint 22

BCNHES

*7. Black

Front 23

Skin Puncture:

Order of Draw

Back 23

1. Bood Gases

2. Slides

3. EDTA microcollection tubes

4. Other microcollection tubes

5. Serum micricollection tubes

Hint 23

BSEOS

Front 24

Tube Color for

Plasma/Lead Testing

Back 24

TAN

Hint 24

Tan (Glass) - Na Heparin

Tan (Plastic) - K2EDTA

Front 25

Specimen for Glucose Testing should be separated from WB w/in

Back 25

30 mins (Henry's)

Hint 25

Bishop: 1 Hr

Front 26

Temperature is _________________ to Draw Volume

Back 26

Inversely Proportional

Front 27

Blood to Anticoagulant Ratio in Blood Bag

Back 27

DOH: 1:7

Harmening: 1:8

Front 28

Tests that uses

Defibrinated Blood

Back 28

1. OFT

2. Hams Acidified Serum Test

3. Auto Hemolysis

Hint 28

OHA

Front 29

Expiration of Evacuated Tubes

Back 29

12 Mos

Hint 29

Sensitization of Evac Tubes: gamma radiation

Front 30

Blood collection for

1. Young children

2. Veins w/ IV Infusion

3. Severely Burned Pxs

Back 30

Skin Puncture

Hint 30

Mixture of venous, capillary, & arterial blood with interstitial and intracellular fluid

Front 31

In Skin Puncture

RBC Cnt, Hct, Hgb, & Plt is __________

WBCs is ____________

Back 31

Lower

Higher

Front 32

Sites for Skin Puncture:

Back 32

Adult: Third (Middle) Finger

Fourth (Ring) Finger

Infants: Lateral (outside) or medial (inside) surface of the plantar side (bottom) of the Heel

Front 33

Puncture Depth

Back 33

Not be more than

2 mm deep

Hint 33

Risk of Bone Injury & Infection (Osteomyelitis)

Front 34

Sharp's Container Color

Back 34

Red

Hint 34

Yellow - Infectious

Yellow w/ Black Band - Chemical

Green - Non-Infectious Wet

Black - Non-Infectious Dry

Orange - Dry Radioactive Wastes

BD - GREEN

NBD - BLACK

Front 35

Most commonly used needles for Veni

Back 35

20, 21, 22 gauge

Standard: 21

Hint 35

Length: 1-1.5 Inches

Front 36

Needle Position & Angle

Back 36

Bevel Up

15 Deg Angle

Front 37

Tourniquet should not be left on the arm for longer than

Back 37

1 min

Hint 37

>1 min Hemoconcentration

*3-4 Inches (Rodak: 2-4) Above Vein Site

Front 38

Pxs w/ IV Infusions at both arms

Back 38

Use Vein below IV site

IV stopped for a minimum of

2 mins before collection

Front 39

Sites for Veni

Back 39

Veins of the Antecubital Region:

1. Median Cubital (Preferred)

2. Cephalic (2nd)

3. Basilic (3rd)

Hint 39

Alternates:

1. Dorsal side of wrist & hand

2. Feet (w/ Physicians Content)

Front 40

Gauge:

Hub Color

Back 40

18 G - Pink

21 G - Green

22 G - Black

23 G - Blue

Front 41

Collection for

New Born Screening

Back 41

Blood Spot Collection

Front 42

Most common complication in Phlebotomy

Back 42

Vascular

Hint 42

1. Bleeding @ site of Puncture

2. Hematoma formation

Front 43

Phleb Complications:

Back 43

1. Vascular

2. Infection

3. Anemia

4. Nuerologic (Seizure/Pain)

5. Cardiovascular

6. Dermatologic

Front 44

Leakage of large amount of blood around puncture site

Back 44

Hematoma

Front 45

Leakage of small amount of blood around puncture site

Back 45

Ecchymosis (Bruise)

Hint 45

Most Common complication in Veni

Front 46

Stress/Anxiety can cause temporary increase in

Back 46

WBCs

Front 47

NPO

Back 47

Non Per Orem

Hint 47

Water is also prohibited

FASTING - can drink water

Front 48

Early Morning Collection

Fasting

No Physical Activity

Back 48

Basal State Collection

Front 49

Effect of Lying Position during Blood Collection

Back 49

Hemodilution

DEC PCV, WBC

Hint 49

Standing: Hemoconc

INC PCV, WBC

Front 50

Decontaminant for Blood Spill

Back 50

Sodium Hypochlorite/Household Bleach

Hint 50

1:10 Dilution

1:9 Ratio

1 parts NaCl: 9 Parts Water

Inactivate:

HBV - 10 mins

HIV - 2 mins

Front 51

Isolation Techniques:

Back 51

1. Strict Isolation

2. Enteric Isolation

3. Respiratory Isolation

4. Wound & Skin Isolation

5. Protective/Reverse Isolation

Front 52

Used when coming in contact with patients who have dysentery and other disorders that spread thru direct contact

Back 52

Enteric Isolation

Hint 52

Gown, Gloves is used

Front 53

Used when the px has infections that are transmitted via droplets or by an airborne route

Back 53

Respiratory Isolation

Hint 53

Gloves, Mask is used

Front 54

Used in cases of skin infection that maybe transmitted directly or indirectly

Back 54

Wound & Skin Isolation

Hint 54

Gown, Gloves is used

Front 55

Require the technologist to protect the px from infection

Back 55

Protective/Reverse Isolation

Hint 55

Gown, Gloves, Mask, Shoe coverings is used

Pxs w/ Leukemia, Severe burns, kidney transplant, body radiation, plastic surgery

Front 56

Used in cases of contagious dses that can be transmitted by direct contact via the air

Back 56

Strict Isolation

Hint 56

Gown, Mask, Gloves is Used