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Platelet Normal Value:

150 - 400 x 10^9/L



150,000 - 400,000 plts/cumm

Platelets <100,000/uL

Abnormally Low

Platelets 30,000-50,000/uL

Bleeding w/ possible trauma

Platelets <30,000/uL

Spontaneous Bleeding Possible

Platelets <5,000/uL

Severe Spontaneous Bleeding

Normal Value for Platelet Adhesiveness (Salzmann)

26-60%

Dec in vWD

Normal Value for Stefanini Method of CRT

Clot Retration Appreciable w/in 1 Hr


Complete w/in 18-24 Hrs

3-5 mL of Blood


Incubation: 37C

Normal Value for MacFarlane Method of CRT

44-67%

5 mL of Blood


Incubation: 37C for 1 Hr

Normal Value for Platelet Estimate

20-80 Plts/OIF

Conversion Factor: 20,000

Common Dilution for Manual Plt Count

1:100

Plts <50 = 1:20


Plts >500 = 1:200



*Phase Contrast Microscope

In Automation, platelets are enumerated as particles w/ size of

2-20 fL

Dec in Platelet Satelitism (Inc WBC Count)

Determine ability of platelets to resist pressure

Capillary Fragility Test/


Tourniquet/


Rumpel Leede's Test

1. Inflate BP Cuff to point halfway b/w systolic & diastolic pressures (never exceed 100 mmHg) maintain pressure for 5 mins


2. Remove BP Cuff and wait 5-10 mins, before counting patechiae

CFT Grading

1+ Few


2+ Many


3+ Multiple


4+ Confluent

Normal Value for Duke Method of Bleeding Time

2-4 Mins

Blot Every 30 Secs

Normal Value for Ivy Method of Bleeding Time

3-6 Mins

Normal Value for Capillary Blood Method of Clotting Time

2-4 Mins

Normal Value for Whole Blood/Lee and White

5-15 Mins

Nornal Value for PT

10-12 Secs

Normal Value for APTT

25-35 Secs

Normal Value for Stypven Time

6-10 Secs

Normal Value for Thrombin Time

10-14 Secs

Normal Value for Reptilase Time

10-15 Secs

Hemoglobin

Birth: 15-20 g/dL


Female: 12-26 g/dL


Male: 13-18 g/dL

Van Slyke Oxygen Capacity Method

1 g Hgb =


1.34 mL of O2

Chemical Mtd for Hgb: Kennedy's, Wong's

1 g Hgb =


3.47 mg Iron

Rule Of Three:

3 x RBC = Hgb


3 x Hgb =


Hct +/- 3%

Rules only apply to RBC of Normal Size & Color

RBC Count NV:

Birth: 5.0-6.5 x 10^12/L



Male: 4.2-6.0 x 10^12/L



Female: 3.6-5.6 x 10^12/L

Dilution if the WBC Count is Above 30.0 x 10^9/L

1:100


1:101

Dilution if WBC Count is


100-300 x 10^9/L

1:200


1:201

Dilution if WBC Count is


Below 3 x 10^9/L

1:10


1:11

Presence of NRBCs

Adult:


>/= 5 NRBCs/100 WBC Diff



Newborn:


>/= 10 NRBCs/100 WBC Diff

WBC Count NV:

Adult:


4.0-11.0 x 10^9/L



At Birth:


30.0 x 10^9/L



Leukocytosis:


>11 X 10^9/L

Platelet Count NV:

150,000-450,000/uL


150-400 x 10^9/L

Retics Count NV:

Adult: 0.5-1.5%


Newborn: 2-6%


Patients Hct Value (%) :


Correction Factor


(Maturation Time in Days)

40-45 = 1


35-39 = 1.5


25-34 = 2


15-24 = 2.5


<15 = 3

Reticulocyte Production Index (RPI) Reference Range:

1 when Hct is 0.45 L/L

Eosinophil Count NV:

50-350 x 10^6/L

MCV NV:

80-100 fL

MCH NV:

27-31 pg

MCHC NV:

31-36%

RDW NV:

11.5-14.5%

*Expressed numerically as the coefficient of variation percentage