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159 Cards in this Set

  • Front
  • Back

In eukaryotic cells, chromosomes are located in _____.

nucleus

On which of the following items could a crime lab use PCR?


saliva- and bloodstained nightshirts


fingernail clippings


vaginal swabs


The first two answers are correct.


The first three answers are correct.

The first three answers are correct

In which of the following places can DNA be found?


chromosomes


a recently used coffee cup


hair follicles


chromosomes and hair follicles


All of the above.

All of the above

You have a segment of DNA with the sequence AATAGCGA. If this segment underwent replication, what is the nucleotide sequence you would see?

TTATCGCT

Gel electrophoresis is best described as a laboratory technique used:

to analyze DNA samples (it separates DNA fragments by size).

A researcher is comparing DNA samples to one derived from a crime scene. To which component of DNA should the researcher pay special attention in order to determine whether a DNA sample matches that of the perpetrator?

the sequence of nucleotides within the DNA

Humans inherit _____ chromosomes from each parent; a male inherits the _____ chromosome from his father.

23; Y

During examination of a gel containing 10 STRs from a heterozygous mother and her biological child, how many bands should be identical in size?

10

DNA regions that do not code for proteins:

can be used to uniquely identify individuals.

Which of the following statements does NOT accurately describe human DNA?Males receive one X chromosome from their mother.Females receive two X chromosomes from their mother.Human DNA is a unique combination of DNA inherited from both parents.Human DNA is located within the nucleus.Humans have 22 pairs of chromosomes and two gender chromosomes.

females receive 2 x chromosomes from their mother

What is the difference between the nucleotides used in DNA replication during the PCR and the nucleotides used during DNA duplication within the cell?

there is no difference; they are the same

DNA profiling with STRs is most accurate when:

many STRs examined

A scientist is looking at a strand of hair under the microscope. Just from this microscopic analysis, what can she determine about the person from which this hair was derived?the texture of the person's hairwhether the person dyes his or her hairthe natural color of the person's hairthe person's ethnicityAll of the answer options are correct.

all options correct

The process of DNA replication requires:breaking of hydrogen bonds.unwinding of the DNA.enzymes.nucleotides.All of the above.

all of the above

Typically, what percentage of people share the same pattern on any one STR site?

5-20

DNA can be visualized as a ladder in which the parallel rungs ("steps") would be:

hydrogen bonded bases

A chromosome is:

a single, large DNA molecule found in the nucleus of human cells.

If the defective gene for color blindness is carried on the X chromosome, and a color blind mother's 23rd pair of chromosomes carries the defect on both chromosomes, what is the likelihood that her son will have color blindness?

100%

A laboratory technique used to replicate, and thus amplify a specific DNA segment, is called:

polymerase chain reaction.

An enzyme that "reads" the sequence of a DNA strand and helps to add complementary nucleotides to form a new strand during DNA replication is:

DNA polymerase.

If you start with one copy of a DNA fragment, how many rounds of PCR will it take to end up with a total of 16 copies?

4

Why is heating the first step in the PCR reaction?

Heating breaks hydrogen bonds between base pairs to separate the DNA strands.

Which of the following is the correct sequence in creating a DNA profile?

collect DNA→extract DNA→PCR to amplify STRs→gel electrophoresis→ analyze gel

Deoxyribonucleic acid (DNA)

hereditary molecule


passed from parents to offspring


common to all living organisms


instruction manual-how to build an individual

Structure of DNA

DNA is composed of four nucleotides


Each nucleotide has 3 components


-1. phosphate group


-2. deoxyribose sugar


-3. nitrogen-containing base

4 Bases in DNA

adenine-thymine


guanine-cytosine

where is DNA found

the nucleus of eukaryotic cells

Endomembrane system: Nucleus

Command center


usually near center


Separated from cytoplasm by nuclear envelope


-consists of double layer of membrane


-nuclear pores permit exchange between nucleoplasm & cytoplasm




Contains chromatin in semifluid nucleoplasm


-chromatin contains DNA of genes, and proteins (histones)


-condenses to form chromosomes


-chromosomes are formed during cell division

Human DNA

one from each pair is inherited from the biological mother


one from each pair inherited from biological father


-23rd chromosome pair determines sex


-XX-female


-XY-male

structure of DNA

composed of nucleotides


-each nucleotides consists of sugar, phosphate, and a base



4 people responsible for figuring out structure of DNA

James Watson


Francis Crick


Maurice Wilkins


Rosalind Franklin

how are double helix's formed

two strands of nucleotides pair up and twist around each other to from a spiral-shaped double helix


-sugars and phosphates form the outside "backbone"


-bases form the internal "rungs"

how are two strands of DNA double helix held together

by base pairing (hydrogen bonding) between the bases of each strand

sequence

the order of nucleotides in a DNA strand


-each individual has a unique DNA sequence


-our uniqueness lies in just 0.1% of our DNA sequence (sequences of any two people are 99.9% identical)


-1 difference in every 1,000 nucleotides


-3 million total nucleotide differences




Differences in sequences can translate to differences in protein function

helicase

unwinding the helix


(backbone of DNA is sugar and phosphate)

DNA replication

natural process by which cells make an identical copy of a DNA molecule




takes advantage of complimentary base pairing rules

DNA replication steps

First, hydrogen bonds that hold base pairs together are broken and the helix is unwound


-DNA polymerase reads the DNA and adds complementary nucleotides using the rules of base pairing




Semi-conservative mechanism


-produces two copies of the original DNA molecule


-each molecule consists of one of the strands of the original DNA molecule and a new strand

The polymerase chain reaction (PCR)

laboratory technique used to replicate and amplify a specific DNA segment




Primers: short segments of DNA that guide DNA polymerase to the section of DNA to copy




allows DNA replication to occur many times


can make billions of copies from a starting sample of just a few molecules of DNA

3 stages of PCR

1. heating


2. strand separation


3. DNA replication

DNA profiling

takes advantage of the fact that no two people have the exact same DNA sequence




Genome


-one complete set of genetic instructions encoded in the DNA of an organism


-Determining sequence of entire genome is extremely time consuming and expensive




Use shortcut:


-use PCR to amplify only specific segments of DNA

Short tandem repeats (STR)

-sections of a chromosome in which DNA sequences are repeated


-Ex: sequence AGCT may be repeated over and over again


-STRs are in the same places along chromosomes


-Exact length of STR varies from person to person

DNA replication?

1. human DNA target


2. DNA primers


3. Taq Enzyme


4. Nucleotides Free


5. Buffer, pH, etc

PCR

Amplifies the DNA nucleotide bonds on a DNA sequence


Helps to determine the unique DNA "fingerprint" of victims or suspects


Short tandem repeats (STR)

Steps of PCR

1.heat to separate the strands


2. PCR primers attach to DNA sequences on either side of the repeats


3. DNA Polymerase copies the sequence


4. process repeated to make many copies

Uses of PCR

Medical applications


Infectious disease applications


Forensic applications


Research applications



Making a DNA profile

1. •Collectcells and extract DNA


2. •UsePCR to amplify multiple STR regions


3. •SeparateSTRs using gel electrophoresis–laboratory technique that separates fragments of DNA by size

making a DNA profile #2

•Separatedfragments of DNA create a specific pattern of bands •Visibleusing fluorescence •Uniqueto each person•Comparepatterns of DNA•Differentindividuals have different DNA banding patterns

DNA ladder

the backbone is made of sugar, phosphate




off of sugar, there are the bases




bases A T and C G




between A & T (two hydrogen base pairs)


between C & G (3 hydrogen base pairs)



Alleles are alternative forms of _____ gene(s) that have ____ nucleotide sequences.

the same; small differences in their

Which of the following individuals with inherited antithrombin deficiency would be most likely to require immediate treatment with antithrombin?

a woman giving birth; a young boy getting surgery

What factors are important in determining the shape of a protein?

hydrophobic amino acid side chains


interactions between amino acid side chains


interactions between amino acid side chains


and waterhydrophilic amino acid side chains

Transcription is the process of copying ____ into _____.

DNA; mRNA

An animal that is transgenic:

contains genes of different species

Why is it beneficial to use a gene that is expressed in mammary glands as a way to produce therapeutic drugs?

Milk is easier to access than blood, and mammary glands produce proteins in relatively large quantities.

Denaturation affects the bonds between amino acids in a protein. What is true of denaturation?

affects the shape of a proteinaffects the function of a proteinmay occur when proteins are heatedmay occur when the pH of a solution changes

Scientists have used genetic engineering technology to clone entire animals.

true



How do you think replacing one amino acid in a protein with another amino acid would affect the shape of that protein?

Changing one amino acid may or may not change the shape of the protein.

What allows different cell types the ability to produce different proteins and therefore have different identities

Only certain cells are able to turn on the regulatory sequence and express certain genes.

Which of the following could be possible if a single nucleotide in the coding sequence of DNA controlling transcription was changed?

the function of the protein could change

Which of the following is true of amino acids?

All amino acids have unique chemical side chains.

Why was it important to use the regulatory sequence of a milk gene when constructing the recombinant gene used to produce human antithrombin?

Proteins expressed in tissues where they shouldn't be can be harmful.


and


Scientists wanted antithrombin produced in milk where it would be easy to harvest.

Which of the following is NOT a mechanism used to prevent milk from transgenic goats from getting into the human food supply?


a double-fenced facility


video cameras


homing devices implanted under the goats' skin


a large bubble over the goats' barn


All of the above.

a large bubble over the goats' barn

Two different proteins have different functions. What MUST be true about these two proteins?

They have different amino acid sequences.They have different three-dimensional shapes.

Which of the following substitutions would you expect to have the greatest impact on the shape of a protein?

substituting a hydrophilic amino acid with a hydrophobic amino acid


substituting a hydrophobic amino acid with a hydrophilic amino acid


reducing the length of the amino acid chain by one amino acid


increasing the length of the amino acid chain by one amino acid

Why is it important to inject a recombinant gene into an embryo instead of an adult?

Injecting the gene into an embryo ensures that all body cells receive a copy of the gene.

Why are many drugs either proteins or drugs that interact with proteins?

Proteins play vital roles within cells which allow cells to function.

If a section of mRNA contains the nucleotide sequence UUGCCUCAC, the sequence of amino acids translated will be:

Leu-Pro-His.

If a mutation in a long chain of codons changes a codon sequence of one codon from CUU to CUC, what affect would that have on the protein produced?

Both of the codons code for the same amino acid and no change would be observed in the protein.

If a section of DNA contains the nucleotide sequence TACCAACGA, the sequence of amino acids translated will be:

Met-Val-Ala.

A tRNA has an anticodon of AGU. If that tRNA carried a trp amino acid, would this result in a normal or abnormal protein?

abnormal

Why do you think bacteria cannot be used to produce complex human proteins, such as antithrombin?

The proteins bacteria produce are not as complex as human proteins.

Which of the following could be an example of a protein?


a hormone


an enzyme


an antibody


a transporter


All of the above are examples.

all of the above are examples

BRCA 1 and 2 gene

-autosomal dominent gene


-bad copy of gene your risk of getting breast cancer is very high

what stages in life are you more likely to get cancer

peak at birth


peak in middle (40's)


shoots up and highest 60+

Why do cells divide?

to replace cells damaged by injury


to maintain healthy tissues


to create a human body during embryonic development


to replace cells that have died

What should normally occur if a problem is detected at a cell cycle checkpoint?

The cell may undergo apoptosis.




The cell may take steps to repair DNA mistakes.

What kind(s) of cells are targeted by chemotherapy?

all dividing cells

What endangered animal lives in the forests where yew trees were being harvested?

northern spotted owls

A biologist measures the amount of DNA in cells growing in a laboratory. She should find that the amount of DNA doubles:

during S phase

If replicated chromosomes failed to condense properly as a cell entered mitosis, what would be a likely outcome?

Chromosomes would become tangled and likely break during mitosis.

Which of the following cells normally divide rapidly and are therefore affected by treatments, such as chemotherapy, that target dividing cells?

intestinal tract, hair follicle, and bone marrow cells.

New chemicals to treat cancer may come from invertebrate animals called sea sponges. Why is it likely that sea sponges produce a cancer treating compound?

They produce chemical defenses to protect against predation.

A tumor is:

a mass resulting from uncontrolled cell division.

The spread of cancer cells from one location in the body to another is known as _____.

metastasis

order of events during the cell cycle:

G1, S, G2, mitosis, and cytokinesis

Which of the following occurs in normal cells, but not in cancer cells?

repair of mistakes in mitosis and apoptosis in damaged cells

What are common side effects of chemotherapy and radiation therapy?

nausea


hair Loss


infections


diarrhea

Cells spend the majority of their time in the cell cycle in ____.

interphase

What will happen in a normal human skin cell if the chromosomes are not aligned for proper chromatid separation during mitosis?

The error will be noticed at a cell cycle checkpoint and the cell will not proceed unless the error is corrected.

How was the supply problem for Taxol solved?

A chemist used a method called semisynthesis to create Taxol using the more common English yew.

Taxol interferes with the normal assembly of the mitotic spindle. How does this affect mitosis?

The cell cannot proceed normally and arrests in metaphase.

A physician is explaining treatment options to a patient who has breast cancer that has undergone metastasis. Which of the following statements might the physician use in explaining the cancer or treatment options?

Chemotherapy should be used to treat breast cancer cells circulating through the body.




Breast cancer cells that have metastasized can develop into tumors in other tissues.




Using Taxol in combination with standard chemotherapies should lead to a longer survival time than standard chemotherapy alone.




All cells undergoing mitosis will be affected by both chemotherapy and radiation.

Which of the following is an example of cells undergoing mitosis?

a new embryo growing




a tree producing new branches




dead skin cells being replaced




scar tissue forming in a wound

What is the most likely mechanism for chemotherapy to cause diarrhea?

The cells lining the intestinal tract are mitotically active cells that divide regularly.

A cell with 46 chromosomes enters the cell cycle. It completes all the stages EXCEPT for cytokinesis, at which point it gets "stuck." How many chromosomes will be present at this point?

92

In a cell with a 24-hour cell cycle, approximately ___% of the time is spent in mitosis and cytokinesis.

10 %

A cell cycle checkpoint should prevent the progression of a cell in which:

DNA bases are paired incorrectly

features of cancer cells?

defective checkpoints and unregulated cell division

What normally occurs when microtubules shorten during mitosis?

Sister chromatids move to opposite ends of the cell

Chemotherapy destroys all dividing cells. Why isn't chemotherapy typically a viable treatment option for pregnant women?

Because cells in the embryo are dividing rapidly, so chemotherapy could stop growth and development of the baby.

Cancer may be caused by:

a checkpoint problem.


failure in apoptosis.


unregulated cell division.


failure in DNA repair mechanisms.

Physical side effects from chemotherapy and radiotherapy could be maximally reduced by:

targeting specific tumor cells

Side effects of chemotherapy―such as vomiting, hair loss, and bruising―occur because:

chemotherapeutic drugs kill both normal and cancerous cells.

apoptosis

the death of cells that occurs as a normal and controlled part of an organism's growth or development.

A 50-year-old woman who was born with a mutation in the gene encoding for a protein responsible for controlling one of the cell cycle checkpoints has developed three additional mutations in the same gene. Which of the following is likely to occur?

She will start to produce cancer cells that divide unregulated.

differences in a comparison of normal cells versus cancer cells?

the number of DNA mutations




the proteins controlling the cell cycle




the density of cells in a tissue




the chemicals secreted by the cells

Tumor suppressor genes produce proteins that help prevent normal cells from dividing uncontrollably by controlling cell division, apoptosis, and DNA repair. Which of the following changes in a cell might lead to cancer?

a decrease in the production of tumor suppressor protein




a lack of tumor suppressor genes

Anemia can be the result of insufficient production of red blood cells. Which tissue is likely to be "at fault" in these cases?

bone marrow

Taxol interferes with the normal organization and function of structures called ____, which are required to _____________.

microtubules; pull sisters apart

A cell has chromosomes that have two sister chromatids. What phase of the cell cycle could this cell be in?

any stage of the cell cycle

If replicated chromosomes failed to condense properly as a cell entered mitosis, what would be a likely outcome?

chromosomes would be tangled and most likely break during mitosis

Over time Taxol tends to become less effective at killing cancer in an individual. What is one suggested reason for why this might be true?

cancer cells develop mutations that change the structure of microtubules

How was Taxol discovered?

A botanist sent plant samples to be screened by the National Cancer Institute.

Duplicated chromosomes are referred to as:

chromatids

A biologist measures the amount of DNA in cells growing in a laboratory. She should find that the amount of DNA doubles:

during S phase

Which of the following might explain why cancer cells divide uncontrollably?

cancer cells overproduce growth signals

Mitosis

the separation and segregation of duplicated chromosomes during cell division

the structure that separates the sister chromatids during mitosis

mitotic spindle

a change in the nucleotide sequence of DNA

mutation

the physical division of a cell into two daughter cells

cytokinesis

hollow protein fibers that are key components of the cytoskeleton and make up fibers of the mitotic spindle

microtubules

proteins located at the centromere that provide an attachment point for microtubules of the mitotic spindle

kinetochore proteins

Why do some people develop cancer at an earlier age than others?

They inherited at least one mutation that affects a tumor-suppressor gene.

What is the normal function of a proto-oncogene?

to help the cell divide appropriately

how a malignant tumor may develop:

A single mutation of BRCA1 is inherited.




An oncogene forms when DNA mistakes are not corrected.




An environmental mutagen such as UV light mutates p53 gene.




Mutation that allows invasion of other tissues develops.

Why would someone with a mutation in the BRCA1 gene have a higher risk of developing cancer earlier in life than someone without a mutation in the BRCA1 gene?

It becomes more likely that additional nonhereditary mutations will occur.

An individual who inherits a mutation in a BRCA1 or BRCA2 gene is at higher risk for developing cancer because these genes are:

tumor-suppressor genes that produce proteins to repair DNA damage.

Which of the following is a normal function of tumor-suppressor genes, such as BRCA1 or p53?

produce proteins that induce apoptosis




produce proteins that repair DNA damage




produce proteins that suppress the cell cycle when there is DNA damage

If you develop a mutation in a skin cell in a key gene involved in regulating the cell cycle, which of the following is/are possible outcomes based on that skin cell mutation?

you may develop skin cancer

Approximately what percentage of women with one copy of a BRCA mutation are expected to be diagnosed with breast cancer by age 50?

30-50 %

Which of the following is true for the younger brother of a 29-year-old woman with breast cancer?

The brother may have an increased risk for prostate cancer.




The brother may not have an increased risk for cancer.




The brother may develop cancer early in life.




The brother may pass a mutated form of a tumor-suppressor gene onto his children.

A cell has an inactivating mutation in one of its two p53 alleles. If this cell sustains DNA damage (e.g., to the HER2 gene), what is likely to happen?

It will undergo apoptosis.




It will pause and repair the DNA damage before dividing.

Which of the following is true of mutations?

They may be advantageous.




They may cause harm.




They create new alleles in a population.

A cell that sustains an activating mutation in a tumor-suppressor gene that makes it always active would be expected to:

stop at checkpoints, even in the absence of DNA damage.

Which of the following behaviors can help reduce the risk of developing mutations?

avoiding exposure to pesticides




not smoking




avoiding meats that have been char-grilled at high temperatures




wearing highly protective sunscreen

Which of the following combinations of mutations is the most likely to convert a normal cell to a cancerous cell?

two mutated p53 alleles, two mutated BRCA1 alleles, one activated allele of HER2

The normal form of the BRCA1 protein is protective because BRCA1 protein:

helps cells repair damaged DNA during the cell cycle

a cancerous tumor that spreads through the body

malignant tumor

a gene that codes for proteins that monitor and check cell cycle progression. when these genes mutate, they lose normal function

tumor suppressor gene

a gene that codes for a protein that helps cell divide normally

proto-oncogenes

In prokaryotic cells gene expression is inherently different than gene expression in eukaryotic cells. Why

Transcription cannot occur in a nucleus in a prokaryotic cell.

a genetically engineered gene

recombinant gene

the process of assembling new genes with novel combinations of regulatory and coding sequences

genetic engineering

a treatment that aims to cure human disease by replacing defective genes with functional ones

gene therapy

the part of the gene that determines the timing, amount, and location of protein production

regulatory sequence

the RNA copy of the original DNA sequence made during transcription

messenger RNA

the set of rules relating particular mRNA codons to particular amino acids

genetic code

a sequence of DNa that contains the info to make at least one protein

gene

a complex of RNA and proteins that carries out protein synthesis in all cells

ribosome

the enzyme that carries out transcription

RNA polymerase

the part of a tRNA molecule that bind to a complementary mRNA codon

anticodon

the part of the gene that specifies the amino acid sequence of a protein

coding sequence

an organism that carries out one or more genes from a different species

transgenic

a visual representation of a person's unique DNA sequence

DNA profile

a single, large DNA molecule wrapped around proteins (located in the nuclei)

chromosome

building block, or monomer, of a nucleic acid

nucleotide

a laboratory technique used to replicate, and thus amplify, a specific DNA segment

polymerase chain reaction

a lab technique that separates fragments of DNA by size

GEL ELECTROPHORESIS

one complete set of genetic instructions encoded in the DNA of an organism

genome