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16 Cards in this Set
- Front
- Back
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transgenic model |
produced by random integration of foreign gene in germ line |
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gene knockout model |
produced by site specific gene insertion |
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history of transgenic mouse |
brinster and palmiter inserted human growth hormone and made gigantic mouse |
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how are genes inserted in mouse genome |
DNA is inserted in diploid zygote. Fertilized eggs are transferred to foster mothers |
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what is the percentage of offspring that will contain foreign DNA from implanted mother |
10-30% |
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how are gene targeting vector introduced to ES cells |
electrophoration or lipid mediated transfection |
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how does the mutated copy of gene get integrated into cell DNA |
homologous recombination upon insertion |
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what is the genotypes of progeny crossed with heterozygenous to heterozygous |
25%WT 50%heterozygous 50% homozygous |
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what happens if homozygote pups are not detected after repeated breeding |
loss of function has most likely resulted in embryo lethality |
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what are some limitations to gene knockout strategy |
1. mutations may give rise to defects that would require more in depth phenotypic research 2. lack of detectable phenotype 3. contribution of defects in multi tissues |
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what needs to happen for a gene to be considered floxed |
2 loxP sites need to be inserted on each side of essential exon |
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define a cre mouse |
mice carry gene for cre recombinase linked to a cell type specific promoter |
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when is Cre protein active in cre-lox mouse |
active in cells that have an active promoter for Cre |
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what happens in the pressence of Cre and recombinatinon in cre-lox mouse |
exon2 deleted and inactivation of target gene |
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exon 2 must be deleted in order for gene to express cre (T/F) |
T |
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females lacking progesteron receptor have |
impaired ovarian functinon impaired uterine functino impaired sexual behavior defective mammary gland development |
