Chapter 9 Review: T-Cell Development

Front 1

What interactions are required for WBC progenitors from BM for T-cell commitment

Back 1

Notch-Notch1

Front 2

T/F The most immature thymocytes are DP

Back 2

F; DN

Front 3

T/F The 2nd stage of thymocyte development is DN, then go onto become SP

Back 3

t

Front 4

What 3 markers define the (DN1-4) and what do they do?

Back 4

CD44, CD25, c-kit

Rearrange TCR B, D, and G

Front 5

T/F alpha rearrangement occurs first in TCR develpment?

Back 5

F; Beta

B selection occurs with invariant TCRa + Cd3

Front 6

What processes is responsible for self tolerance

Back 6

Positive Selection

Front 7

What process is responsible for Self-MHC restriction

Back 7

Negative selection

Front 8

T/F Low/Intermediate-affinity signals lead to apoptosis

Back 8

F; positive selection and onto SP status

Front 9

95% of Thymocytes do not interact with _____ expressed by thymic epithelium and die by neglect

Back 9

MHC/Self peptide

Front 10

What determines whether or not a DP becomes CD4 vs CD8

Back 10

signal continuity:

Continuous interaction with MHC2 --> CD4

Interrupted MHC1 --> CD8 (CD8 is down-regualted so Interrupted)

Front 11

What TF is expressed in medulla but not cortext and what is it for?

Back 11

AIRE; lets Thymic tissue express tissue-specific Ag for T cell selection/Removal

Front 12

Death by neglect occurs because:

Back 12

No sufficient TCR signaling

Front 13

Extrinsic pathway

Back 13

Activated death receptors

Front 14

Intrinsic pathway

Back 14

Mitochondrial Cyt C release

Front 15

T/F BH3 family have only Pro-Apop factors

Back 15

T; Bim/Bad/Puma

Front 16

T/F Bcl-2 has both Anti and Pro Apop factors

Back 16

T;

Pro is BAX/BAK

Anti are the Bcls

Front 17

Beta selection initiates progression to ____ (5)

Back 17

DN4 stage

Proliferation

Allelic exclusion

Progression to DP stage

And TCR alpha rearrangment

Front 18

Neg selection occurs in the _____

Back 18

medulla of thymus

Front 19

Why do most thymocytes die in the thymus?

Back 19

1) Do not make viable TCR

2) Do not bind self-MHC

Front 20

Cytochrome C is in intrinsic or extrinsic pathway?

Back 20

Intrinsic

Front 21

T/F Bcl2 inhibits apop by inhibiting Bax

Back 21

F; inhibits Cyt C release

Front 22

Do g/d TCR require MHC? If not what are consequnces

Back 22

no; able to bind non peptides

similar to innate IS recognition

Front 23

What kind of CD4/8 cells are in Thymus of normal mice? RAG1/2 KO?

Same for LN?

Back 23

1. DN, CD4+, CD8+, DP
2. DN
3. DN CD4+, CD8+
4. DN

Front 24

What kind of Thymocytes are in mice that don't have MHC 2

Back 24

Everything but CD4 SP;

DN, DP, CD8+

Front 25

What kind of Thymocytes are in mice that don't have AIRE

Back 25

All stages but there would be self-reactive cells

Front 26

What kind of Thymocytes are in mice that don't have TCR alpha

Back 26

DN and DP only; cant positively select for A/B

Will have some gamma delta

Front 27

What kind of thymocytes wouldn't stain for CD3 and TCRB

Back 27

DNs

Front 28

What kind of thymocytes wouldn't stain for TCRb but do for CD3?

Back 28

G/D T cells

Front 29

T/F When MHC and TCR match lysis occurs, and doesn't occur when they differ

Back 29

T; ignore other MHCs

Front 30

T/F Only TC with mature TCR express CD3

Back 30

F; Pre-TCR also do