The purpose of this experiment is to determine the pH of known substances by use of a pH color indicator of red cabbage and the use of a pH meter. As well as, to observe the pH changes in a substance by adding in more basic of acidic to buffered solutions and unbuffered solutions. This experiment will also will show how to calculate H3O+, OH- and pH by use of known values. In order to do this lab, there are a few key components one needs to know and understand first.…
Three drops of each liquid were put onto into the spots on the spot plate (with pH paper). 4. The color of the pH paper was noted to determine the pH of each substance. 5. A result of the pH level of each substance was recorded in an observation chart.…
MR test tube inoculated and incubated for 24 hours, ten to fifteen drops of methyl red indicator then added to the cultured. If it turned red then that was the positive for the presence of mixed acids. No color change was negative, however, if the media turned orange, it was inconclusive. VP test tube was inoculate and incubated up to 3 days, vortexed, added Barrits Reagent A (alpha-napthol) and Barrits Reagent B (potassium hydroxide). Red or pink color was positive reaction, no color change was negative, however it could take up to an hour to see the color change.…
The purpose of this lab was to measure the exact mass of a primary standard, to find the precise number of moles of potassium hydrogen phthalate (KHP) included to the reaction, the molarity of NaOH at the equivalence point, the molarity of an aqueous solution containing NaOH, as well as how much volume was needed to get to the equivalence point specified by the end point. The first step in this experiment was to prepare a solution of NaOH with an unknown molar concentration. Then, determine the concentration of the NaOH by titration, using KHP which has a known concentration and is considered a primary standard. during this experiment, Phenolphthalein was found to be the best indicator for the titration of vinegar with NaOH, due to it having…
Compare both sets of pH to determine which had an easier time resisting change. C. Titration curves. 1. Insert NaOH into burette, proceed to clamp burette to stand and insert into beaker containing 10 mL of HCl. 2.…
This will help us determine our concentration of NaOH. Phenolphthalein is an indicator that changes color to light pink when it reaches the end point. Phenolphthalein is used because the titration of weak acid and strong base has a basic pH end point. KHP is used as the primary standard because it is solid, easy to weigh to reduce errors, stable when exposed to air and the molar ratio between NaOH and KHP…
Discussion In this study, the Catechol enzyme was studied under the conditions of varying pH, temperature, substrate concentration, and enzyme concentration. In Figure 1, the data suggested that the trend was neither directly nor inversely proportional, but the highest activity rate was at 24°C. Most enzymes denatured at higher temperatures of approximately 40°C, which led to the inability to see any color change (Helms et al., 1998). At lower temperatures, the enzyme was somewhat efficient because molecules move slower at lower temperatures, so enzymes lost productivity.…
It is important to note that the goal of this experiment was to find the pKa value of bromothymol blue, a pH indicator commonly used in titrations. [UNC, 2017] The pKa is where the number of moles of acid to conjugated base are equivalent. To find this, both Beer’s Law and spectroscopy were utilized in an attempt to make graphs to show just where this value occurred. According to Sigma-Aldrich, the pKa of bromothymol blue is approximately 7.10.…
Reported Value for Sodium hydroxide Molarity: 0.07605 M ± 6.07E-05 M (average) (standard deviation) Table 2: TITRATION OF AN UNKNOWN SAMPLE CONTAINING POTASSIUM ACID PHTHALATE Sample Trial 1 2 3 Mass of the POTASSIUM ACID PHTHALATE unknown (to 0.0001 g) 0.5028 0.5165 0.5263 0.5394 Final volume of Sodium hydroxide (to 0.01 mL) 15.55 30.70 16.11…
Throughout this acid, base and buffer lab, this will allow each individual to gain an enhanced sense of knowledge of how these different aqueous solutions work as a buffer system or how they are unable to perform as buffer system mixed with two solutions together. This can be identified through the colour change to measure pH behaviour in several aqueous solutions, therefore the different factors that will cause the change of pH. This will gain understanding of how this relates to the biological studies of biology and to the advantage of humans. Hydrochloric acid combined with water will not a make good buffer system. Hydrochloric acid is a strong acid that will not coexist with any other base in equilibrium.…
The pH indictor for this test is phenol red. Phenol red turn yellow in a acidic enviroment and pink is alkaline enviornment. This test is useful for enterics bacteria like the genus Proteus. Unknown bacteria #20 has the following results: Catalase positive, MSA negative, Nutrient gelatin negative, nitrate reduction positive, SIM positive, and Urease positive. After review these results, the unknown was determing to be Proteus.…
Purpose The purpose of this experiment is to find the pKIn blue using spectrophotometry. Bromophenol blue dissociate in water by the following reaction: i) HIn + H2O ⇋ H3O+ + In- The KIn value can be calculated from the reaction: ii) K_In=[H3O+]…
For the second titration, at step two 25 cm3 of gastric juice was used instead of hydrochloric acid. The pH of the gastric juice was measured using a pH meter. Results Table 1 Titration of 25 cm3 sodium hydroxide solution with 0.01 mol dm-3 hydrochloric acid Initial burette reading (cm3) Final burette reading (cm3) Volume Used (cm3) Rough Titration 0.00 25.0 25.0 First Accurate Titration 25.0 50.0 25.0 Second Accurate Titration 0.00 25.1 25.1…
Scientists use a scale to measure how acidic or basic a liquid is. That scale is called a pH scale. The pH scale is a number from zero to fourteen. Accordingly, zero to seven liquids are considered acid. However, seven is considered a neutral, like distilled water.…
Racheal A. Krasner Professor Garfield Beckford CHEM 1211 L 25 September 2015 Lab Report Concerning Acid-Base Titrations The purpose of this experiment was to recognize the procedures for conducting acid-base titrations. Each acid-base titration used one solution to analyze another; it also involved the use of an indicator, which denoted the endpoint of the titration. The indicator used was phenolphthalein; it produced a pink hue when the titrations reached their endpoints – between pH 8 and pH 10.…