The Role Of I-Kappa-B-Alpha

1530 Words 7 Pages
I-Kappa-B-alpha which is often abbreviated IkBa is a protein whose role is to mask the nuclear localization signals to inhibit the NF-kB (nuclear factor kappa beta) proteins by keeping them in inactive states and isolating them in the cytoplasm, this inhibits the NF-kB transcription factors and blocks the NF-kB transcription factors from binding with DNA. NF-kB is a protein complex that is responsible for controlling cytokine production, transcription of DNA, cell survival, and plays a key role in regulating the body’s immune response to infection. It is important for IkBa is be present in cells and carry out its role because incorrect regulation of the NF-kB protein complex has been linked to cancer, viral infections, inflammatory diseases, …show more content…
The cell is stimulated by immune and proinflammatory responses. IkBa undergoes phosphorylation, which promotes ubiquitination and degradation, this enables the dimeric RELA to move to the nucleus and activate transcription. IkBa is involved in rocky mountain spotted fever, an infection caused by rickettsia rickettsia, a bacterium that is carried by a certain species of ticks and can spread to humans if they are bit an infected tick. This infection induces a two-phase pattern of NF-kB that is activated in cultured human endothelial cells and is characterized by an early short-term phase at 3 h and a late long-term phase at 18 to 24 h. To determine and clarify the process of this disease, the expression of NF-kB subunits p65 and p50, IkB proteins, IkBalpha, and IkBbeta were investigated by researchers. The investigation revealed that the transcript and protein levels of p65, p50, and IkBbeta remained unchanged during the infection but Ser- 32 phosphorylation of IkBalpha at 3 h had a significant increase in uninfected cells. There was also an increase in the expression of IkBalpha in mRNA which returned to normal over time. The catalytic subunits of IkB kinase (IKK) complex, IKKalpha and IKKbeta, are measured by vitro kinase assays with immunoprecipitates from uninfected endothelial cells and rickettsia rickettsia infected endothelial cells. These measurements revealed a large increase at 2 h after infection. The activation phase of IKK and a beginning phase of NF-kB response are inhibited by treatment with heat and are completely terminated by formalin fixation of rickettsia. Parthenolide and aspirin are IKK inhibitors that block the activities

Related Documents