The purpose of the Determining Food Dye Content Lab was to identify the food dye coloring in green apple Gatorade, and determine the dye’s concentration to ultimately recreate a solution containing the same concentration of dyes as the original. To determine the concentration of the dyes present in the green apple Gatorade, a spectrometer was used to measure the wavelengths and absorbance values for the given dyes. A sample of the green apple Gatorade was tested in the spectrometer to measure its absorption and compare the values collected to stock dyes provided in lab. The data displayed that the green apple Gatorade contained blue-1 food dye because the Gatorade’s wavelength at peak one was 422.3 Nm, and blue-1 food dye’s peak one was 423.7+-.1.5…
The Spec 20 was set to 600 nm and % transmittance was measured of the standard solutions. Absorbance was calculated and recorded. Then, 2 g of solid CuSO4∙5H2O was measured out and placed in a 100 mL beaker. Water was added (about 15 mL) to make a 20 mL of 0.5 M solution from CuSO4∙5H2O by. Solution…
The Chobani Greek had an absorbance of .160 nm, and the Yoplait had an absorbance of .118 nm. The data for the standards created a standard curve, which was used to determine the concentrations. Interpolations were taken to determine the concentrations of the samples. The concentration of the Chobani Greek was about .16 mg/mL, and was about .05 mg/mL for the Yoplait sample (Figure…
Betacyanin pigments from beta vulgaris vacuoles were use in this experiment to determine the various effects of diffusion into its surrounding environment: 60%detergent, which is shown in the figures below. The four figures below are a set of data that were collected and analyzed the effects of time, salt concentration and ionic compositions on the rate of diffusion. Both figure one and two show a set of measurements between two different salt concentrations representing their absorbance versus time. Both figures one and two have six trend-lines that are all deceleration curves. In figure one, at the initial time of 3minutes, the six data points are relatively close to each other and are overlapping.…
Beer’s law indicates that the relationship between absorbance and concentration is linear; the absorbance can be found on the vertical axis, and the corresponding intercept on the plotted line determines the concentration. The peak absorbance (λmax) , as measured by the Ocean Optics Spectroscopy, and the known concentration values for each of the four solutions created were plotted for each food dye. Once the dyes were identified and the concentrations of each dye determined, a sample solution was created using the same concentration. The concentration of Blue 1 was determined to be 2.014 x 10-6 mol/L.…
From here, the scientist utilized the colorimeter to find the absorbance of this solution in collusion with the absorbance plot for Copper (II) Nitrate solutions of various molarities. From the linear fit of the Beer’s Law plot, calculations for the determination of the molarity and percent mass of copper were executed. Because the absorbance and concentration variables have a direct relationship in the Beer’s Law Equation, the linear fit was an accurate method for calculating the unknown. From the graph, it was observed that as the concentration (or molarity) of the Copper (II) Nitrate solution increased, the absorbance of the solution increased. The scientist was able to relate this finding back to her conclusions from the previous activity to realize that the dark the shade of blue, the less color variance that was reflected and the higher the absorbance.…
Figure eight initially, however, was made from the table found in figure 2. The absorption values were found by taking the absorption value for the various solutions at the maximum wavelength value found from the highest pH solution…
Scientists have the ability to determine the molarity and the contents of a substance by measuring the intensity of the light that is emitted through the solution. Scientists accomplish this by using the technique of spectroscopy; spectroscopy can be defined as the science affiliated with the spectra produced when matter interacts with light. Lab 7 investigation 19, “What Factors Affect the Intensity of Color?” expands on the process of spectroscopy, and its relation to the molarity of a substance. The goal of this lab was to determine the effects of the intensity of color of a solution (2). Spectroscopy is frequently used in the medical field by using the process of infrared spectroscopy, which is used to examine biological fluids.…
Through calculation, the percent transmittance allowed for the determination of absorbance. To zero-out the spectrophotometer, about 2/3 of the blank solution was poured into a cuvette and placed in the spectrophotometer device until it displayed 100% (allowed for the measurement of the absorbance of just the phosphate). The solution in the cuvette was disposed in a designated beaker, then it was thoroughly rinsed with distilled water and dried with Kimwipes. About 2/3 of the first solution was poured into the cleaned cuvette and placed in the spectrophotometer for testing. The previous steps were repeated for each of the remaining solutions.…
Through this experiment we measured how fast a chemical reaction occurs, by changing the degradation rate in Albumin when added to different enzymes. Albumin is a protein found in egg white, which is considered to have important storage and nutritional functions. Albumin has also been used in medicine to treat heavy metal intoxication. We ran Albumin through four different conditions to see which would make the Albumin degrade faster. We predicted that the Albumin would degrade with pepsin the fastest since it is essential for digestion of substances in the stomach.…
The optimal wavelength of maximum cranberry juice absorption was determined to be 525 nm. The calibration curve could then be created by measuring the absorbance of known concentrations, 5% to 25%, of cranberry juice at 525 nm, and generating a calibration curve of absorbance vs concentration. The absorbance of the cranberry apple juice was then measured at the wavelength of maximum absorbance. Absorbance is a measure of the amount of light that passes through a sample at a selected wavelength. The absorbance is measured by the spectrophotometer 20 which functions by emitting a visible light source through the sample.…
M2V2 must be change into (M_1 V_1)/M_2 =V_2. The final volume needed for the red dye was 5.00 mL, the blue dye 3.60 mL, and the rest was filled up with deionized water up to 25 mL in a volumetric flask. Only one trial was done. To measure the accuracy of the created solution, the solution was put in a UV-Vis spectrometer. Then the absorbance curve of the created solution was compared to the absorbance curve of the Gatorade.…
The lowest reaction rate was at temperature zero degrees celsius, the peak absorbance around 0.2-0.3…
By using the pseudo first-order kinetics method, you are able to determine the rate law for the reaction between crystal violet and hydroxide ion. crystal violet hydroxide CV+ + OH- ————> CVOH Rate=k[CV+]m [OH-]n The objective for this experiment was to analyze the absorbance of the dyed molecule crystal violet using a colorimeter and the molar absorbance of the reactant. This data was used to calculate the concentration of the the crystal violet and hydroxide ions.…
From the absorbance of sample A3 and B3 and their diluted versions, the following graphs can be drawn: Figure 2- A graph showing the relation between concentration of bromophenol blue in M and their absorbance for the acidic form of the molecule, showing the molar absorptivity constant of the molecule at wavelengths 440 nm and 590 nm. The reading with concentration 6 x 10-4 M at wavelength 440 nm was discarded as it is too off from the trend line that it is not following the trend at all.…