Experimental
At the outset of the experiment, the available food dyes were identified as:
FD&C Blue #1
FD&C Blue #2
FD&C Green #3
FD&C Red #3
FD&C Red #40
FD&C Yellow #5
FD&C Yellow #6
Given that the food dye was of a purple color, a hypothesis was formed that the color profile contained either FD&C blue #1 or blue #2 and red #3 or red #40. To determine which dyes were present, a spectroscopic reading was taken of the purple Gatorade using an Ocean Optics Spectrometer, connected to a computer via Go Link!, and the peak absorbance and wavelengths were recorded and compared to the wavelengths of each sample dye. The closest matches were FD&C Red #40 and FD&C Blue #1, as discussed in the results and discussion sections of this paper. …show more content…
Beer’s law indicates that the relationship between absorbance and concentration is linear; the absorbance can be found on the vertical axis, and the corresponding intercept on the plotted line determines the concentration. The peak absorbance (λmax) , as measured by the Ocean Optics Spectroscopy, and the known concentration values for each of the four solutions created were plotted for each food dye.
Once the dyes were identified and the concentrations of each dye determined, a sample solution was created using the same concentration. The concentration of Blue 1 was determined to be 2.014 x 10-6 mol/L. The concentration of red 40 was determined to be 1.078 x 10-5 mol/L. To prepare a solution of these concentrations, 10 mL of Blue #1 and 12 mL of Red #40 were combined in a beaker, and the beaker was then filled to the 100 mL mark.
To evaluate the accuracy of this synthesized color profile, Ocean Optics Spectrometer was used to evaluate the wavelength and absorbance values for this synthesized solution. After the solution was prepared, it was evaluated using the Ocean Optics Spectrometer. The peak absorbance versus concentration was then graphed according to Beer’s Law (M1V1=M2V2) to verify if the synthesized solution was indeed an accurate color match for the purple Gatorade
At the outset of the experiment, the available food dyes were identified as:
FD&C Blue #1
FD&C Blue #2
FD&C Green #3
FD&C Red #3
FD&C Red #40
FD&C Yellow #5
FD&C Yellow #6
Given that the food dye was of a purple color, a hypothesis was formed that the color profile contained either FD&C blue #1 or blue #2 and red #3 or red #40. To determine which dyes were present, a spectroscopic reading was taken of the purple Gatorade using an Ocean Optics Spectrometer, connected to a computer via Go Link!, and the peak absorbance and wavelengths were recorded and compared to the wavelengths of each sample dye. The closest matches were FD&C Red #40 and FD&C Blue #1, as discussed in the results and discussion sections of this paper. …show more content…
Beer’s law indicates that the relationship between absorbance and concentration is linear; the absorbance can be found on the vertical axis, and the corresponding intercept on the plotted line determines the concentration. The peak absorbance (λmax) , as measured by the Ocean Optics Spectroscopy, and the known concentration values for each of the four solutions created were plotted for each food dye.
Once the dyes were identified and the concentrations of each dye determined, a sample solution was created using the same concentration. The concentration of Blue 1 was determined to be 2.014 x 10-6 mol/L. The concentration of red 40 was determined to be 1.078 x 10-5 mol/L. To prepare a solution of these concentrations, 10 mL of Blue #1 and 12 mL of Red #40 were combined in a beaker, and the beaker was then filled to the 100 mL mark.
To evaluate the accuracy of this synthesized color profile, Ocean Optics Spectrometer was used to evaluate the wavelength and absorbance values for this synthesized solution. After the solution was prepared, it was evaluated using the Ocean Optics Spectrometer. The peak absorbance versus concentration was then graphed according to Beer’s Law (M1V1=M2V2) to verify if the synthesized solution was indeed an accurate color match for the purple Gatorade