Abstract— Monitoring hemodynamic responses in rodent stroke model is important in studying of ischemic stroke. However, it is hard for single imaging modality to comprehensively explore these changes. In this study, we implemented laser speckle imaging (LSI) and visible-light optical coherence tomography (Vis-OCT) to detect cerebral blood flow (CBF), angiography and oxygen saturation (sO2) in the distal middle cerebral artery occlusion (dMCAO) model. LSI provides full-field, real-time cerebral blood flow (CBF) map to guide the Vis-OCT imaging. Vis-OCT offers angiography and sO2 measurements. The results showed the detailed CBF and vasculature changes before and after dMCAO. After dMCAO, sO2 changed little in arteries and arterioles, while sO2 decreased in veins and venules. We also showed that larger veins had a higher decrease of sO2 value in consecutive vein branches after dMCAO. It is suggested that combined LSI/Vis-OCT provides a promising tool for the rodent stroke model study.
Ischemic stroke accounts for 87% of all stroke cases and is a leading cause of death and disability worldwide . In humans, the majority of stroke lesions located in the middle cerebral artery (MCA) territory . The mouse distal middle cerebral artery occlusion (dMCAO) model mimics a major mechanism of cortical strokes in humans and provides a practical approach to study focal ischemia .
Perturbed vascular morphology impairs the normal blood supply and oxygen delivery in…