S. Coli Lab Report

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Azziz reported that the hexane extract of S.aromaticum has good antibacterial properties on both E.coli, and S. aureus based on the zone of inhibition which has a diameter range of 7mm to 10mm. In this study, the extract of hexane of S.aromaticum showed positive antibacterial properties against E. coli, and S. aureus for concentrations of 25, 50, 75, 100. The methanol extract showed positive against S. aureus for 75 and 100 mg/ml. The DCM extract showed positive for E. coli, S. aureus, (100 mg/ml). The comparison of the three extracts of clove oil shows that the hexane extract of clove oil has the highest zone of inhibition with the least concentration. This experiment indicates that the major chemical compound of S.aromaticum, Eugenol, shows …show more content…
López, C. Sánchez, R. Batlle, and C. Nerín from Spain in 2005, they conducted the antimicrobial activity of clove on several gram negative bacterium E. Coli, Yersinia enterocolitica,Salmonella choleraesuis, Pseudomonas aeruginosa, and several gram positive bacterium, S.aureus, Bacillus cereus, Entero faecalis, and Listeria monocytogenes. The clove essential oil was supplied by ARTIBAL. The compounds in clove essential oil was identified using the database of gas chromatography ion trap mass spectrometer (GC-IT-MS). The antimicrobial activity test was performed using solid diffusion assays vapor diffusion assay and MIC after incubation period. Solid diffusion assay was performed by inoculating 100μL of the solution with E.coli and S. aureus into the respective petri dish. The clove essential oil in the filter disk was placed onto the cultured media. P. López, C. Sánchez, R. Batlle, and C. Nerín reported that the clove essential oil which contained Eugenol has antimicrobial activity against S. aureus and E.coli. Based on Table 3 and Figure 4, the total inhibitory measured in solid diffusion array for S. aureus is higher than E.coli. The MIC value represents the lowest concentration of essential oil to inhibit the growth of microorganism. The MIC values for both bacteria is 26.6 μL/L which showed inhibition of

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