Qualitative Phytochemical Analysis Of CBE And CQE

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3. Results and discussion
3.1. Qualitative and quantitative phytochemical analysis
Preliminary phytochemical screening of the aqueous extract of both CBE and CQE showed the presence of alkaloids, flavonoids, reducing sugar, phenols, steroids like terpenoids and saponins (Table 1), whereas tannins are absent. Table 2. shows the quantitative analysis of phytochemicals of CBE and CQE. The concentration of phyto-constituents varied between the plants and also within the plant. The total steroid content was found to be higher in CBE followed by total phenols, flavonoids and ascorbic acid. On the other hand, in CQE steroids are higher in concentration compared to all other phyto-constituents followed by flavonoids, total phenolics and ascorbic acid.
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Among these compounds higher level of phytosteroids were observed in both the plants, further flavonoids were higher in CQE and phenolic compounds were higher in CBE. In fact, phytosterols, phenolics and flavonoids were reported to be powerful free radical scavengers (Chanda and Dave, 2009). These phyto-constituents exhibit medicinal and also the physiological activities of any plant material (Sofowra, 1993). Phyto-constituents alone determine the total antioxidant activities of a plant in the form of phenolic compounds such as flavonoid, phenolic acids, phytosteroids, vitamin C, carotenoids, tocopherols etc., (Ali et al., …show more content…
The study reveals that the antioxidant activity of the extracts was an increasing trend with the increasing concentration. Both plants showed similar effect compared with ascorbic acid standard (Table 5). In the present study, the in vitro antioxidant assays exhibited that both plant extracts scavenged the free radicals in a dose dependent manner and the activity was compared to the standards such as BHT and ascorbic acid. The increased antioxidant activity in CBE and CQE extracts are due to the presence of high concentration of phenolic acids and flavonoids. The redox properties of phenolics and flavonoids chelate the metals and quenching the singlet oxygen. The total phenolic concentration showed close correlation with increased antioxidant activity, because, plant phenolics present in fruit and vegetables have received considerable attention for potential antioxidant properties (Lopez-Velez et al., 2003). There is a linear correlation between phenolic content and antioxidant activity (Gheldof and Engeseth, 2002). High concentration of total phenolics present in both plant extracts showed their total antioxidant potentiality through the in vitro antioxidant assays. However, CBE showed a relatively higher scavenging property than CQE. These data are in accordance with others, who have shown that high total phenolic content increases the antioxidant activity (Holasova et al.,

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