How Escherichia Coli Bacteria, And Make It Ampicillin Resistant

722 Words Nov 28th, 2015 3 Pages
The purpose of this experiment is to determine is to be able to successfully transform Escherichia coli Bacteria, and make it ampicillin resistant when exposed to an extracellular plasmid of DNA. It involves introducing the E. coli and allowing it to adapt to the DNA of a new environment. The E. coli must be placed in a tube, into an ice bath, causing the cell to shrink. From there it is transferred to a preheated bath, where the cell is heat shocked, allowing the plasmids ' to enter the cell at a rapid pace, once the cell expands. After the tubes are incubated, they were transferred to six agar plates. There were two types of agar plates, one included the plasmid lux and the control plasmid pUC18. Every agar plate was labeled independently LBC , LB/AMP C , LB NP , LB lux , LB/AMP lux , and LB/AMP NP. Before placed onto the agar plates, we needed to sterilize the cell spreader, in order to avoid contaminating the E. coli. After the cell spreader is sterile, a dropper is used to put the E. coli onto the plate, and then use the cell spreader to spread out the E. coli for an even coat. From there, the agar plates are left alone until the liquid from each plate is absorbed, and then incubating them once again. After allowing them to be incubated, two types of growth were able to be seen on the plate, each one indicating whether it was able to become ampicillin resistant.

Transformation of cells is a widely used tool in genetic engineering, and is of importance in molecular…

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