Introduction:
Genetic transformation is used in many areas of biotechnology. In medicine, diseases caused by defective genes are beginning to be treated by gene therapy by genetically transforming a sick person’s cells with healthy copies of the defective gene that causes their disease. Genes can be cut out of human, animal, or plant DNA and placed inside bacteria, which could treat a person with that disease. For example, a healthy person’s gene for the insulin can be put into bacteria. Under the right conditions, the bacteria can make useable human insulin. This insulin can then be used to treat patients with the genetic disease or diabetes.Therefore knowing how to properly insert genes into bacteria could solve many medical issues humans …show more content…
In the plate labelled -pGLO with LB/amp, there is no bacteria that grew because ampicillin was available. In the plate labelled +pGLO with LB/amp, there are about 100 bacterias estimated with a clear color. Finally, in the plate labelled +pGLO with LB/amp/ara, there are about 450+ bacterias with a glowing green color because the addition of arabinose allowed the bacteria to inhibit the ability to glow. Because the bacteria was glowing green on the Lb/amp/ara plate, we were able to transform …show more content…
I hypothesized that in the plate labeled +pGLO LB/amp, colonies will grow and survive. In the plate labeled +pGLO with amp/LB/ara, bacteria will grow and glow green under UV light. In the plate labeled -pGLO with LB/amp, no bacteria will be able to grow. Finally, in the last plate labeled -pGLO with LB and no amp, will host a lawn of colony.To test out my hypothesize, I, along with my class, ingusted in the pGLO lab (the precedure is above). The results were that in the plate labeled +pGLO LB/amp, colonies will grow and survive. In the plate labeled +pGLO with amp/LB/ara, bacteria will grow and glow green under UV light due to the addition of arabinose. In the plate labeled -pGLO with LB/amp, no bacteria will be able to grow due to the bacteria not being resistant to the antibiotic. Finally, in the last plate labeled -pGLO with LB and no amp, will host a lawn of colony due to no limiting factors. This results conformed my hypothesizes. Because the experiment was very timed, the smallest time error can create a mistake in the overall results. Also, an error could occur if the loops are not changed which could cause contamination in the plate therefore affecting the result. To further explore this topic, one question one can ask him or herself is “How is changing the amount of µl added going to affect the result of the
Genetic transformation is used in many areas of biotechnology. In medicine, diseases caused by defective genes are beginning to be treated by gene therapy by genetically transforming a sick person’s cells with healthy copies of the defective gene that causes their disease. Genes can be cut out of human, animal, or plant DNA and placed inside bacteria, which could treat a person with that disease. For example, a healthy person’s gene for the insulin can be put into bacteria. Under the right conditions, the bacteria can make useable human insulin. This insulin can then be used to treat patients with the genetic disease or diabetes.Therefore knowing how to properly insert genes into bacteria could solve many medical issues humans …show more content…
In the plate labelled -pGLO with LB/amp, there is no bacteria that grew because ampicillin was available. In the plate labelled +pGLO with LB/amp, there are about 100 bacterias estimated with a clear color. Finally, in the plate labelled +pGLO with LB/amp/ara, there are about 450+ bacterias with a glowing green color because the addition of arabinose allowed the bacteria to inhibit the ability to glow. Because the bacteria was glowing green on the Lb/amp/ara plate, we were able to transform …show more content…
I hypothesized that in the plate labeled +pGLO LB/amp, colonies will grow and survive. In the plate labeled +pGLO with amp/LB/ara, bacteria will grow and glow green under UV light. In the plate labeled -pGLO with LB/amp, no bacteria will be able to grow. Finally, in the last plate labeled -pGLO with LB and no amp, will host a lawn of colony.To test out my hypothesize, I, along with my class, ingusted in the pGLO lab (the precedure is above). The results were that in the plate labeled +pGLO LB/amp, colonies will grow and survive. In the plate labeled +pGLO with amp/LB/ara, bacteria will grow and glow green under UV light due to the addition of arabinose. In the plate labeled -pGLO with LB/amp, no bacteria will be able to grow due to the bacteria not being resistant to the antibiotic. Finally, in the last plate labeled -pGLO with LB and no amp, will host a lawn of colony due to no limiting factors. This results conformed my hypothesizes. Because the experiment was very timed, the smallest time error can create a mistake in the overall results. Also, an error could occur if the loops are not changed which could cause contamination in the plate therefore affecting the result. To further explore this topic, one question one can ask him or herself is “How is changing the amount of µl added going to affect the result of the