Scientific Article Analysis Questionnaire

1572 Words 7 Pages
Joshua K. Westervelt
Dr. Fair
MCB 4404
Scientific Article Analysis Questionnaire
A. Read the abstract to get an overview of the article.
B. Read the entire article and write notes from each section (Introduction/ Materials-Methods/ Results/ Discussion). Always refer back to the abstract if you get lost in the main sections of the article.
C. Then answer following questions (also see “Explanation of Article Sections” below).

1. Who did the research, and where?
The research was conducted by Sana S. Dastgheyb et al. at the National Institute of Health in Bethesda, Maryland, in association with the Dept. of Orthopedic Surgery, at Thomas Jefferson University, in Philadelphia, Pennsylvania.

2. A. What
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In TSB, the WT organism formed no agglomerates, while Δagr and Δpsm developed a small number. In SF, all organisms developed large agglomerates. The data is also visualized by microscopy in Fig. 4 (B). Researchers concluded that the lack of statistical difference between the WT and mutants in SF proves that the lack of PSM is responsible for the development of exceptionally large agglomerates.
Immunoblotting with PIA-specific antibodies revealed that PIA was present in higher quantities in SF than in serum or TSB, and that its presence is diminished with the introduction of PSMs. This technique showed a significantly higher level of PIA in the WT organism in SF than in TSB. These findings correlate with the Cellometer and microscopy findings. In the SF, where host proteins are present, large agglomerates are observed. However, in the TSB, PSMs are free to develop and no agglomerates are found. This could indicate that the host proteins, such as fibrin, are a trigger for the down-regulation of
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This is the section where the authors review prior research related to the study that they are conducting and identify potential gaps in the knowledge base which justify their new research.
When summarizing, state what is already know about the topic and state what aspects the new research is going to contribute (or what knowledge gaps it is trying to fill.)
Materials/ Methods
This is the section where the authors describe how the experimental part of the research was conducted.
When summarizing, describe what, where and how it was done.
This is where the authors present and analyze the collected data and draw their conclusions.
When summarizing, you do not have to explain about the statistical analysis, just how the authors arrived at their main conclusion from the data.
This is where the authors expound on what their research means in the grand scheme of existing knowledge, draw more conclusions, and often (not always) discuss how the research could potentially be expanded.
When summarizing, state how the research contributed to already existing

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